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Circ_RPPH1 regulates glioma cell malignancy by binding to miR-627-5p/miR-663a to induce SDC1 expression

Wei Chen1, Xiao Yu1, Ning Wang1

  • 1Department of Neurosurgery, The First Affiliated Hospital of Xi'an Jiao-Tong University, No.227, Yanta west Road, Xi'an, 710061, Shaanxi province, China.

Metabolic Brain Disease
|March 25, 2022
PubMed
Abstract

Insights

Circular RNA RPPH1 (circ_RPPH1) promotes glioma progression by upregulating Syndecan 1 (SDC1) via sponging miR-627-5p and miR-663a. Knockdown of circ_RPPH1 inhibits glioma cell malignancy and tumorigenesis, suggesting its potential as a therapeutic target.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Circular RNAs (circRNAs) are increasingly recognized for their roles in cancer progression.
  • The specific function of circRNA ribonuclease P RNA component H1 (circ_RPPH1) in glioma remains largely uncharacterized.
  • Understanding circ_RPPH1's role is crucial for developing novel glioma therapies.

Purpose of the Study:

  • To investigate the role of circ_RPPH1 in glioma cell malignancy.
  • To elucidate the molecular mechanisms underlying circ_RPPH1's function in glioma.
  • To assess the therapeutic potential of targeting circ_RPPH1 in glioma.

Main Methods:

  • Quantitative real-time PCR and Western blot to assess expression levels of circ_RPPH1, miR-627-5p, miR-663a, and SDC1.
  • Cellular assays including MTT, flow cytometry, EdU, colony formation, transwell migration, and invasion assays.
  • Dual-luciferase reporter assays to confirm interactions between circRNAs and miRNAs, and in vivo mouse models to evaluate therapeutic efficacy.

Main Results:

  • Circ_RPPH1 and SDC1 expression were significantly elevated in glioma tissues and cells, while miR-627-5p and miR-663a were downregulated.
  • Circ_RPPH1 knockdown suppressed glioma cell proliferation, migration, and invasion, and induced apoptosis, both in vitro and in vivo.
  • Circ_RPPH1 directly interacted with miR-627-5p/miR-663a, regulating SDC1 expression and mediating glioma cell behaviors.

Conclusions:

  • Circ_RPPH1 knockdown inhibits glioma tumorigenesis by downregulating SDC1 through binding to miR-627-5p/miR-663a.
  • Circ_RPPH1 acts as a molecular sponge for miR-627-5p and miR-663a, promoting glioma progression.
  • Circ_RPPH1 represents a promising therapeutic target for glioma treatment.
Keywords:
GliomaSDC1circ_RPPH1miR-627-5pmiR-663a

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