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Related Concept Videos

Notch Signaling Pathway03:14

Notch Signaling Pathway

4.5K
The Notch signaling pathway is a major intracellular signaling pathway that is highly conserved over a broad spectrum of metazoan species. It stands unique from other intracellular signaling mechanisms in animals because notch protein itself acts as the receptor as well as the primary signaling molecule.
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not...
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Role Of Notch Signalling In Intestinal Stem Cell Renewal01:12

Role Of Notch Signalling In Intestinal Stem Cell Renewal

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Notch signaling was first discovered in Drosophila melanogaster, where it is involved in cell lineage differentiation. Notch signaling regulates the maintenance and differentiation of intestinal stem cells or ISCs by controlling the expression of atonal homolog 1 or Atoh1. Atoh1 directs cells to differentiate into secretory cells.
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
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mTOR Signaling and Cancer Progression03:03

mTOR Signaling and Cancer Progression

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The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
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Non-Canonical Wnt Signaling Pathways01:41

Non-Canonical Wnt Signaling Pathways

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Wnt is a zygotic effect gene that is expressed during very early embryonic development. It regulates various processes in animals starting from early development through the adult stage, such as organogenesis in the embryo and maintenance of neuronal and blood stem cells. Wnt proteins can induce a wide variety of intracellular pathways depending upon the specific abilities of different Wnt ligands to form a complex with shared and cognate receptors in the presence of different co-receptors. The...
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Protein Glycosylation01:25

Protein Glycosylation

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Glycosylation, the most common post-translational modification for proteins, serves diverse functions. Adding sugars to proteins makes the proteins more resistant to proteolytic digestion. Glycosylated proteins can act as markers and receptors to promote cell-cell adhesion. Additionally, they have many essential quality control functions in the cell, such as correct protein folding and facilitating transport of misfolded proteins to the cytosol, which can be degraded.
Glycosylation occurs in...
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Canonical Wnt Signaling Pathway02:54

Canonical Wnt Signaling Pathway

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The gene encoding the main signaling molecules of the Wnt signaling pathways (the Wnt proteins) was discovered almost four decades ago by Nüsslein-Volhard and Wieschaus. They identified and originally named the gene "wingless" (wg) after a phenotype discovered during their landmark genetic screen in Drosophila for body pattern defects. At around the same time, another researcher named Harold Varmus found that a murine tumor virus activates the mammalian wg homolog, Int-1, which...
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Updated: Sep 29, 2025

Integration of Bioinformatics Approaches and Experimental Validations to Understand the Role of Notch Signaling in Ovarian Cancer
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Significant Roles of Notch O-Glycosylation in Cancer.

Weiwei Wang1, Tetsuya Okajima1,2, Hideyuki Takeuchi1,3

  • 1Department of Molecular Biochemistry, Nagoya University School of Medicine, 65 Tsurumai, Showa-ku, Nagoya 466-8550, Japan.

Molecules (Basel, Switzerland)
|March 26, 2022
PubMed
Summary

Notch signaling regulates cell development and its dysregulation is linked to cancer. O-glycosylation modifies Notch receptors, impacting signaling pathways crucial for cancer progression.

Keywords:
EGF repeatO-glycosylationcancernotch signaling

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Stimulation of Notch Signaling in Mouse Osteoclast Precursors
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Cell Aggregation Assays to Evaluate the Binding of the Drosophila Notch with Trans-Ligands and its Inhibition by Cis-Ligands
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Cell Aggregation Assays to Evaluate the Binding of the Drosophila Notch with Trans-Ligands and its Inhibition by Cis-Ligands

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Stimulation of Notch Signaling in Mouse Osteoclast Precursors
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Cell Aggregation Assays to Evaluate the Binding of the Drosophila Notch with Trans-Ligands and its Inhibition by Cis-Ligands
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Area of Science:

  • Cell Biology
  • Molecular Biology
  • Cancer Research

Background:

  • Notch signaling is vital for cell development and differentiation.
  • Dysregulation of Notch signaling contributes to various human cancers, with context-dependent oncogenic or tumor-suppressive roles.
  • Aberrant receptor/ligand expression and altered trafficking impact Notch signaling, potentially leading to tumorigenesis.

Purpose of the Study:

  • To review the fundamental aspects of Notch signaling.
  • To present current knowledge on Notch receptor O-glycosylation based on structural classification.
  • To elucidate the regulatory role of O-glycosylation in Notch signaling within the context of cancer.

Main Methods:

  • Literature review focusing on Notch signaling pathways.
  • Analysis of structural data on Notch receptor O-glycosylation.
  • Synthesis of information on O-glycosylation's impact on Notch signaling in cancer.

Main Results:

  • Notch signaling plays diverse roles in different cancers.
  • Three primary types of O-linked modifications (O-glucosylation, O-fucosylation, O-N-acetylglucosamine) occur on Notch EGF repeats.
  • O-glycosylation influences ligand-receptor binding and trafficking, thereby regulating Notch signaling.

Conclusions:

  • O-glycosylation is a key modulator of Notch signaling.
  • Understanding Notch receptor O-glycosylation provides insights into cancer development and progression.
  • Targeting O-glycosylation pathways may offer novel therapeutic strategies for cancer.