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Chick embryonic erythrocyte antigen--tissues sharing expression.

M M Miller, J R Pink

    Experimental Cell Research
    |December 1, 1986
    PubMed
    Summary
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    A novel glycoprotein antigen, chickEE, previously found on embryonic chicken red blood cells, is now identified in various adult tissues. This discovery necessitates a re-evaluation of the chickEE antigen's function in cell surfaces.

    Area of Science:

    • Cell Biology
    • Immunology
    • Developmental Biology

    Background:

    • An erythrocyte surface glycoprotein antigen (Mr 48 kD) is present in embryonic and young chickens but absent in adults.
    • This antigen's differential expression has been linked to hematopoietic tissue maturation.

    Purpose of the Study:

    • To investigate the expression of the erythrocyte antigen in various embryonic and adult chicken tissues.
    • To characterize the antigen using a monoclonal antibody (maEE1) and 2D gel electrophoresis.

    Main Methods:

    • Monoclonal antibody maEE1 production and characterization.
    • Two-dimensional (2D) gel electrophoresis of tissue extracts.
    • Immunofluorescent labeling of cryosections and flow cytometry.

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    Main Results:

    • The chickEE glycoprotein antigen is expressed in multiple tissues beyond erythrocytes, including retina, muscle, liver, epithelia (gut, kidney), and lymphoid organs in both young and adult chickens.
    • 2D gel patterns of chickEE from various embryonic and adult tissues closely resemble that of embryonic erythrocytes.
    • Monoclonal antibody maEE1 shows specific reactivity with chickEE across these diverse tissues.

    Conclusions:

    • The chickEE antigen is widely expressed across various tissues and developmental stages in chickens.
    • The function of the chickEE glycoprotein on cell surfaces requires re-evaluation due to its broad expression.