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Published on: July 27, 2016
Oxidative stress-related circulating miRNA-27a is a potential biomarker for diagnosis and prognosis in patients with
Yingwei Ou1, Rongcheng An1, Haochu Wang2
1Department of Emergency, Zhejiang Provincial People's Hospital (Affiliated People's Hospital, Hangzhou Medical College), 158 Shangtang Road, Hangzhou, 310014, Zhejiang, People's Republic of China.
Background:
Oxidative stress plays a critical role on the processes of sepsis, and several microRNAs have been identified that may regulate the occurrence of oxidative stress. However, the relation between oxidative stress-related microRNA 27a (miR-27a) and sepsis is unknown. The present study aimed to determine the value of circulating miR-27a for the diagnosis and prognosis of sepsis.
Methods:
This retrospective study included 23 patients with sepsis and 25 without sepsis treated at the emergency intensive care unit (EICU) or our institution between January 2019 and January 2020. Levels of circulating miR-27a and levels of oxidative stress-related indicators were measured and compared between sepsis and non-sepsis patients. Receiver operating characteristic (ROC) curve analysis was used to determine diagnostic efficiency of miR-27a.
Results:
Circulating miR-27a levels in sepsis patients were higher than those in non-sepsis patients (p < 0.05), and levels were significantly higher in patients that died than those that lived (p < 0.05). In patients with sepsis, circulating miR-27a level was positively correlated with serum malondialdehyde (MDA) level (rs = 0.529, p = 0.007), and negatively correlated with serum glutathione peroxidase (GSH-Px) level (rs = - 0.477, p = 0.016). No significant correlation was observed between circulating miR-27a and serum superoxide dismutase (SOD) in sepsis patients (rs = - 0.340, p = 0.096). The area under the ROC curve (AUC) of miR-27a level for prediction of sepsis was 0.717 (p = 0.009) and for 28-day mortality was 0.739 (p = 0.003).
Conclusions:
This study showed that circulating miR-27a level is correlated with oxidative stress and mortality in patients with sepsis, and may serve as a potential non-invasive molecular biomarker.
Insights
Circulating microRNA 27a (miR-27a) levels are elevated in sepsis patients and correlate with oxidative stress. Higher miR-27a indicates increased mortality risk, suggesting its potential as a sepsis biomarker.
Area of Science:
- Biochemistry
- Molecular Biology
- Critical Care Medicine
Background:
- Oxidative stress is a key factor in sepsis pathogenesis.
- MicroRNAs (miRNAs) are implicated in regulating oxidative stress.
- The specific role of microRNA 27a (miR-27a) in sepsis was previously undetermined.
Purpose of the Study:
- To investigate the diagnostic and prognostic value of circulating miR-27a in sepsis.
- To determine the relationship between miR-27a, oxidative stress markers, and patient outcomes.
Main Methods:
- Retrospective analysis of 23 sepsis and 25 non-sepsis patients.
- Measurement of circulating miR-27a and oxidative stress indicators (MDA, GSH-Px, SOD).
- Receiver operating characteristic (ROC) curve analysis for diagnostic and prognostic efficiency.
Main Results:
- Circulating miR-27a levels were significantly higher in sepsis patients compared to controls.
- miR-27a levels were elevated in non-survivors versus survivors.
- miR-27a positively correlated with malondialdehyde (MDA) and negatively with glutathione peroxidase (GSH-Px).
- ROC analysis showed miR-27a had diagnostic value for sepsis (AUC=0.717) and 28-day mortality (AUC=0.739).
Conclusions:
- Circulating miR-27a is associated with oxidative stress and mortality in sepsis.
- miR-27a shows potential as a non-invasive biomarker for sepsis diagnosis and prognosis.
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