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Approach to an efficient pretreatment method for rice straw by deep eutectic solvent for high saccharification

Premeshworii Devi Maibam1, Arun Goyal2

  • 1School of Energy Sciences and Engineering, Indian Institute of Technology Guwahati, Guwahati 781039, Assam, India.

Bioresource Technology
|March 26, 2022
PubMed
Summary

Deep eutectic solvent pretreatment effectively removed lignin from rice straw, significantly increasing cellulose content and surface area. This optimized process enhances rice straw biomass for efficient saccharification.

Keywords:
Deep eutectic solventDelignificationPretreatment efficiencyRice strawSaccharification efficiency

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Area of Science:

  • Biomass Pretreatment
  • Green Chemistry
  • Biorefining

Background:

  • Rice straw is an abundant lignocellulosic biomass.
  • Efficient pretreatment is crucial for unlocking its potential.
  • Deep eutectic solvents offer a sustainable pretreatment option.

Purpose of the Study:

  • To optimize deep eutectic solvent pretreatment of rice straw.
  • To evaluate the impact of choline chloride:acetic acid ratio, temperature, and time.
  • To assess the effect on lignin removal and carbohydrate retention.

Main Methods:

  • Central Composite Design (CCD) for process optimization.
  • Deep eutectic solvent comprising choline chloride (ChCl) and acetic acid (AA).
  • Analysis of lignin removal, total carbohydrate content (TCC), and structural properties.

Main Results:

  • Optimized conditions: 1:3.59 ChCl:AA molar ratio, 126°C, 150 min.
  • Achieved 83.1% delignification and 83.7% pretreatment efficiency.
  • Increased cellulose content to 0.73 g/g, 31% higher crystallinity, and 17-fold surface area increase.

Conclusions:

  • Optimized ChCl:AA pretreatment significantly enhances rice straw structure.
  • Pretreated biomass shows improved cellulose enrichment and accessibility.
  • High saccharification efficiency (92.2%) achieved, yielding 18.8 g/L total reducing sugar.