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Growth of the ICRC bacilli in the foot-pad of mice
Abstract:
The ICRC bacilli are acid-fast bacilli cultivated from M. leprae isolates of lepromatous tissue. The ICRC bacilli from C-44 in the conditioned medium were subjected to foot-pad test, in both normal and T/900r mice. The bacilli exhibits a limited multiplication in normal mice while a continuous growth in T/900r mice. The maximum yield for normal and T/900r mice was 10(7) and 10(9)/foot-pad, respectively. The infiltration of voluntary muscle tissue as the main localization site was common for both normal and T/900r mice with evidence of dissemination in the latter. The spread of AFB to sciatic nerve, induction of liver granuloma and the foot-drop was observed only in T/900r mice. These experiments show that the growth of ICRC bacilli in mouse foot-pad is very similar to that of M. leprae confirming a test for identification.
Insights
The ICRC bacilli, cultivated from leprosy tissue, show limited growth in normal mice but continuous multiplication in T/900r mice. This mouse foot-pad test confirms ICRC bacilli identification, mirroring Mycobacterium leprae behavior.
Area of Science:
- Microbiology
- Immunology
- Infectious Diseases
Background:
- Leprosy is caused by Mycobacterium leprae.
- ICRC bacilli are acid-fast bacilli isolated from lepromatous tissue.
Purpose of the Study:
- To evaluate the growth and dissemination of ICRC bacilli in mouse foot-pads.
- To determine if the ICRC bacilli foot-pad test can be used for identification.
Main Methods:
- ICRC bacilli were inoculated into the foot-pads of normal and T/900r mice.
- Bacterial multiplication and tissue infiltration were assessed.
- Dissemination, granuloma formation, and clinical signs were observed.
Main Results:
- ICRC bacilli exhibited limited multiplication in normal mice (10^7) and continuous growth in T/900r mice (10^9).
- Muscle tissue infiltration occurred in both mouse types.
- Dissemination to sciatic nerve, liver granuloma, and foot-drop were observed exclusively in T/900r mice.
Conclusions:
- The growth pattern of ICRC bacilli in mouse foot-pads closely resembles that of Mycobacterium leprae.
- The mouse foot-pad test is a viable method for identifying ICRC bacilli.