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OPTIMIZING EXTRACTION AND CONCENTRATION METHODS FOR LC-MS/MS ANALYSIS OF VETERINARY PHARMACEUTICALS IN EQUINE-ASSOCIATED ENVIRONMENTAL MATRICES.

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Influence of humic acids on the passive sampling of pharmaceutical compounds by POCIS and DGT.

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Implementation of an easy and cost-effective method for POCIS in situ calibration using o-DGT for pharmaceutical compounds.

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Development of a multi-hormone analysis method by LC-MS/MS for environmental water application using diffusive

Rachel Martins de Barros1, Sophie Lissalde1, Robin Guibal2

  • 1University of Limoges, PEIRENE, URA IRSTEA, 123 Avenue Albert Thomas, 87060, Limoges Cedex, France.

Talanta
|March 26, 2022
PubMed
Summary

A new liquid chromatography tandem mass spectrometry method efficiently monitors hormones in water using passive sampling. The technique ensures accurate hormone detection and stability, crucial for environmental water quality assessment.

Keywords:
Hormone analysisHormones storage stabilityLiquid chromatography-electrospray ionisation-triple quadrupoleMatrix effectsPassive samplingo-DGT

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Area of Science:

  • Environmental Chemistry
  • Analytical Chemistry
  • Water Quality Monitoring

Background:

  • Hormones are critical environmental contaminants.
  • Efficient water monitoring methods are needed to detect and quantify hormones.

Purpose of the Study:

  • To develop and validate an efficient water analysis method for four hormone families.
  • To assess hormone stability under various storage conditions.
  • To evaluate matrix effects and apply the method for river water monitoring.

Main Methods:

  • Liquid chromatography tandem mass spectrometry (LC-MS/MS) with triple quadrupole coupling.
  • Passive sampling using o-DGT (diffusive gradient in thin-film sampler).
  • Analysis according to French Standard NF T90-210.

Main Results:

  • A single-run method for 13 hormones with a calibration range of 0.1–20 μg/L.
  • Hormone stability confirmed for up to 60 days under specific storage conditions.
  • Successful application in river water monitoring, detecting Estrone and Androstenedione at ng/L levels.

Conclusions:

  • The developed LC-MS/MS method is efficient and accurate for water monitoring of hormones.
  • Passive sampling coupled with LC-MS/MS provides reliable environmental monitoring data.
  • The method is suitable for assessing hormone contamination in aquatic ecosystems.