Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Differential Impact of Intraocular Pressure Lowering on Glaucoma Progression in African and European Descent Individuals.

American journal of ophthalmology·2026
Same author

Image-Quality-Aware Multimodal Artificial Intelligence for Automated Structured OCT Report Generation in Glaucoma Evaluation.

Ophthalmology science·2026
Same author

Glaucomatous Remodeling of the Lamina Cribrosa: Association With Visual Field Progression.

Investigative ophthalmology & visual science·2026
Same author

A Comparative Analysis of Visual Field Test Results Using Tablet-Based and Table-Mounted Devices in a Telemedicine Program Set in Underserved Areas.

Translational vision science & technology·2026
Same author

Dysfunction of α2δ4 leads to photoreceptor degeneration through disrupted synaptic mitochondria and calcium crosstalk.

Cell death & disease·2026
Same author

Macula Spatial Patterns and Their Association With Central Visual Field Progression in Glaucoma Using Artificial Intelligence.

Journal of glaucoma·2026

Related Experiment Video

Updated: Sep 29, 2025

Second Harmonic Generation Signals in Rabbit Sclera As a Tool for Evaluation of Therapeutic Tissue Cross-linking TXL for Myopia
12:25

Second Harmonic Generation Signals in Rabbit Sclera As a Tool for Evaluation of Therapeutic Tissue Cross-linking TXL for Myopia

Published on: January 6, 2018

7.9K

Scleral crosslinking using genipin can compromise retinal structure and function in tree shrews.

Mustapha El Hamdaoui1, Alexander M Levy2, Aaron B Stuber1

  • 1Department of Ophthalmology and Visual Sciences, University of Alabama at Birmingham, Birmingham, AL, USA.

Experimental Eye Research
|March 27, 2022
PubMed
Summary

Genipin for myopia control showed potential but repeated retrobulbar injections caused retinal damage in tree shrews. Lower doses and improved delivery are needed for safe and effective scleral crosslinking.

Keywords:
ElectroretinographyGenipinMyopiaOptical coherence tomographyScleral crosslinking

More Related Videos

Scleral Cross-linking Using Riboflavin and Ultraviolet-A Radiation for Prevention of Axial Myopia in a Rabbit Model
05:56

Scleral Cross-linking Using Riboflavin and Ultraviolet-A Radiation for Prevention of Axial Myopia in a Rabbit Model

Published on: April 3, 2016

9.0K
A Model of Glaucoma Induced by Circumlimbal Suture in Rats and Mice
07:00

A Model of Glaucoma Induced by Circumlimbal Suture in Rats and Mice

Published on: October 5, 2018

10.6K

Related Experiment Videos

Last Updated: Sep 29, 2025

Second Harmonic Generation Signals in Rabbit Sclera As a Tool for Evaluation of Therapeutic Tissue Cross-linking TXL for Myopia
12:25

Second Harmonic Generation Signals in Rabbit Sclera As a Tool for Evaluation of Therapeutic Tissue Cross-linking TXL for Myopia

Published on: January 6, 2018

7.9K
Scleral Cross-linking Using Riboflavin and Ultraviolet-A Radiation for Prevention of Axial Myopia in a Rabbit Model
05:56

Scleral Cross-linking Using Riboflavin and Ultraviolet-A Radiation for Prevention of Axial Myopia in a Rabbit Model

Published on: April 3, 2016

9.0K
A Model of Glaucoma Induced by Circumlimbal Suture in Rats and Mice
07:00

A Model of Glaucoma Induced by Circumlimbal Suture in Rats and Mice

Published on: October 5, 2018

10.6K

Area of Science:

  • Ophthalmology
  • Biomedical Engineering
  • Animal Models

Background:

  • Scleral crosslinking with genipin is a potential myopia control strategy.
  • Genipin's efficacy in animal models is known, but its ocular safety is not well-established.
  • Understanding genipin's safety profile is crucial for its clinical translation.

Purpose of the Study:

  • To investigate the safety of genipin-induced scleral crosslinking via retrobulbar injections.
  • To evaluate the impact of genipin on retinal structure and function in a myopia model.
  • To determine safe dosage and delivery methods for genipin in tree shrews.

Main Methods:

  • Juvenile tree shrews received retrobulbar injections of genipin (0 mM, 10 mM, 20 mM) or sham.
  • Form deprivation myopia was induced in the injected eye.
  • Retinal function (electroretinography), structure (OCT, histology) were assessed.

Main Results:

  • Genipin injections caused retinal nerve fiber layer thinning, even at low doses.
  • Electroretinography showed impaired retinal function (b-wave changes) in genipin-treated eyes.
  • Histology revealed photoreceptor and retinal pigment epithelium degeneration at higher genipin doses.

Conclusions:

  • Repeated retrobulbar genipin injections at 10 mM or higher are unsafe in tree shrews.
  • Genipin shows potential for myopia control, but requires safer, sustained delivery strategies.
  • Caution is advised when considering genipin scleral crosslinking for human myopia treatment.