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Updated: Jul 26, 2026
ELIME Enzyme Linked Immuno Magnetic Electrochemical Method for Mycotoxin Detection
Published on: October 23, 2009
The evolution of multiplex detection of mycotoxins using immunoassay platform technologies
Saowalak Adunphatcharaphon1, Christopher T Elliott2, Thanasat Sooksimuang3
1School of Food Science and Technology, Faculty of Science and Technology, Thammasat University, 99 Mhu 18, Pahonyothin Road, Khong Luang, Pathum Thani 12120, Thailand; International Joint Research Center on Food Security, 113 Thailand Science Park, Phahonyothin Road, Khong Luang, Pathum Thani 12120, Thailand.
Accurate, affordable, and rapid multiplex detection methods are needed to address co-contamination by multiple hazardous mycotoxins. This review covers emerging immunoassays for detecting mycotoxins in agricultural products and feed.
Area of Science:
- Food Safety and Toxicology
- Analytical Chemistry
- Biotechnology
Background:
- Mycotoxins pose significant risks to public health, economy, and environment.
- Co-contamination of multiple mycotoxins in commodities and environment exacerbates these risks.
- Need for accurate, affordable, and rapid multiplex detection methods is critical.
Purpose of the Study:
- To review emerging rapid immuno-based multiplex methods for mycotoxin detection.
- To cover methods published within the last five years for agricultural products and feed ingredients.
- To discuss principles, pros, cons, and performance of these assays.
Main Methods:
- Review of scientific literature on rapid multiplex immunoassays.
- Discussion of various immunoassay formats: lateral flow, fluorescence polarization, chemiluminescence, surface plasmon resonance, surface enhanced Raman scattering, electrochemical sensors, and nanoarrays.
- Analysis of assay principles, advantages, disadvantages, and performance metrics.
Main Results:
- Overview of diverse rapid immuno-based multiplex detection strategies for mycotoxins.
- Comparative analysis of different assay technologies regarding sensitivity, specificity, speed, and cost-effectiveness.
- Identification of key performance characteristics for each discussed method.
Conclusions:
- Emerging sensor technologies, nanomaterial-based signals, and advanced sample preparation are crucial for future mycotoxin detection.
- Future trends point towards quantitative analysis capabilities and integration of diverse sensor platforms.
- Continued development of rapid, multiplex immunoassays is essential for effective mycotoxin management.
