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Updated: Sep 28, 2025

Quantification of Endogenous Auxin and Cytokinin During Internode Culture of Ipecac
Published on: March 15, 2018
Measuring Endogenous GA and IAA
Yunping Chen1, Zhiwu Dan1, Shaoqing Li1
1State Key Laboratory of Hybrid Rice, Key Laboratory for Research and Utilization of Heterosis in Indica Rice of Ministry of Agriculture, College of Life Sciences, Wuhan University, Wuhan, China.
Abstract:
Plant hormones regulate many physiological processes that largely influence growth, differentiation, and development. Contents of phytohormones were analyzed using a liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS) system. This protocol describes a detailed procedure to extract and quantify indole-3-acetic acid (IAA) and gibberellin acid (GA) in rice (Oryza sativa) tissues using high-performance liquid chromatography (HPLC)-based method.

