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Updated: Sep 28, 2025

Three-Dimensional Bone Extracellular Matrix Model for Osteosarcoma
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TPX2 regulated by miR-29c-3p induces cell proliferation in osteosarcoma via the AKT signaling pathway

Dongsheng Zhu1, Xiangfei Xu1, Ming Zhang1

  • 1Department of Pediatric Surgery, The First People's Hospital of Lianyungang Affiliated to Xuzhou Medical University, Lianyungang, Jiangsu 222000, P.R. China.

Oncology Letters
|March 28, 2022
PubMed

Insights

Targeting protein for Xenopus kinesin-like protein 2 (TPX2) is upregulated in osteosarcoma, correlating with poor prognosis. MicroRNA-29c-3p inhibits osteosarcoma cell proliferation by downregulating TPX2, suggesting potential as a therapeutic target.

Area of Science:

  • Oncology
  • Molecular Biology
  • Biochemistry

Background:

  • Osteosarcoma is a primary bone malignancy with limited treatment options.
  • Understanding the molecular mechanisms driving osteosarcoma progression is crucial for developing novel therapies.
  • Targeting protein for Xenopus kinesin-like protein 2 (TPX2) has emerged as a potential factor in cancer development.

Purpose of the Study:

  • To investigate the role of TPX2 in osteosarcoma.
  • To explore the relationship between TPX2 and microRNA-29c-3p (miR-29c-3p) in osteosarcoma.
  • To evaluate TPX2 and miR-29c-3p as potential prognostic biomarkers for osteosarcoma.

Main Methods:

  • Analysis of The Cancer Genome Atlas (TCGA) database for TPX2 expression and survival data.
  • Quantitative PCR to assess TPX2 expression in osteosarcoma tissues.
  • Luciferase reporter assays and TargetScan to confirm the interaction between TPX2 and miR-29c-3p.
  • Kaplan-Meier survival analysis to correlate TPX2 levels with patient prognosis.
  • In vitro experiments using small interfering RNA (siRNA) against TPX2 in osteosarcoma cell lines to assess proliferation (MTT assay) and AKT pathway activation (Western blot).

Main Results:

  • TPX2 expression was significantly upregulated in osteosarcoma tissues compared to normal tissues.
  • High TPX2 expression was associated with poorer patient survival.
  • miR-29c-3p was found to directly target and downregulate TPX2 expression.
  • Knockdown of TPX2 using siRNA inhibited osteosarcoma cell proliferation and affected the AKT signaling pathway.
  • miR-29c-3p expression was downregulated in osteosarcoma.

Conclusions:

  • TPX2 is overexpressed in osteosarcoma and linked to unfavorable prognosis.
  • miR-29c-3p acts as a tumor suppressor by targeting and inhibiting TPX2 in osteosarcoma.
  • The miR-29c-3p/TPX2 axis influences osteosarcoma cell proliferation via the AKT pathway.
  • Both TPX2 and miR-29c-3p show potential as prognostic indicators for osteosarcoma.
Keywords:
AKT pathwayTPX2osteosarcomapediatricsproliferation

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