POD Nanozyme optimized by charge separation engineering for light/pH activated bacteria catalytic/photodynamic...

Area of Science:

• Biomedical Engineering
• Materials Science
• Nanotechnology

Background:

• Nanocatalysts show promise for anti-infection therapy but are limited by insufficient reactive oxygen species (ROS) generation, particularly against drug-resistant bacteria.
• Developing efficient nanozymes with enhanced catalytic activity is crucial for overcoming limitations in clinical anti-infection treatments.

Purpose of the Study:

• To synthesize and characterize a novel Ag/Bi2MoO6 (Ag/BMO) nanozyme optimized for enhanced anti-bacterial activity.
• To investigate the mechanisms underlying the nanozyme's efficacy, including its peroxidase-mimicking activity, NIR-II photodynamic performance, and ion release characteristics.
• To evaluate the in vivo therapeutic potential of the Ag/BMO nanozyme against drug-resistant bacterial infections.

Main Methods:

• Solvothermal reaction and photoreduction were employed to synthesize the Ag/BMO nanozyme.
• Charge separation engineering was utilized to optimize photoactivated peroxidase-like activity and NIR-II photodynamic performance.
• Theoretical calculations were performed to elucidate the electronic structure and charge transfer dynamics within the nanozyme.

Main Results:

• The synthesized Ag/BMO nanozyme demonstrated significant bactericidal performance against methicillin-resistant Staphylococcus aureus (MRSA), achieving ~99.9% eradication.
• The enhanced antibacterial efficacy was attributed to the synergistic effects of peroxidase-like activity, NIR-II photodynamic therapy, and acidity-enhanced Ag+ release.
• In vivo studies showed promising therapeutic effects of Ag/BMO nanoparticles in treating MRSA-infected wounds.

Conclusions:

• Ag/BMO nanozymes, optimized via charge separation engineering, offer a potent strategy for combating drug-resistant bacterial infections.
• The combination of multiple catalytic mechanisms and stimuli-responsive ion release significantly enhances nanozyme antibacterial performance.
• This work provides a rational design strategy for developing advanced nanozymes for biomedical applications, particularly in anti-infection therapy.