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Screening of Potential Key Biomarkers for Ewing Sarcoma: Evidence from Gene Array Analysis
Duming Zhong1, Dan Chen1, Guangquan Zhang1
1Department of Orthopedics, The Fourth Affiliated Hospital of Nanchang University, Nanchang, People's Republic of China.
Background:
Ewing's sarcoma (ES) is a common bone cancer in children and adolescents. There are ethnic differences in the incidence and treatment effects. People have made great efforts to clarify the cause; however, the molecular mechanism of ES is still poorly understood.
Methods:
We download the microarray datasets GSE68776, GSE45544 and GSE17674 from the Gene Expression Omnibus (GEO) database. The differentially expressed genes (DEGs) of the three datasets were screened and enrichment analysis was performed. STRING and Cytoscape were used to carry out module analysis, building a protein-protein interaction (PPI) network. Finally, a series of analyses such as survival analysis and immune infiltration analysis were performed on the selected genes.
Results:
A total of 629 differentially expressed genes were screened, including 206 up-regulated genes and 423 down-regulated genes. The pathways and rich-functions of DEGs include protein activation cascade, carbohydrate binding, cell-cell adhesion junctions, mitotic cell cycle, p53 pathway, and cancer pathways. Then, a total of 10 hub genes were screened out. Biological process analysis showed that these genes were mainly enriched in mitotic nuclear division, protein kinase activity, cell division, cell cycle, and protein phosphorylation.
Conclusion:
Survival analysis and multiple gene comparison analysis showed that CDCA8, MAD2L1 and FANCI may be involved in the occurrence and prognosis of ES. The purpose of our study is to clarify the DEG and key genes, which will help us know more about the molecular mechanisms of ES, provide potential pathway or targets for the diagnosis and treatment.
Insights
This study identifies key genes involved in Ewing sarcoma (ES), a childhood bone cancer. Findings highlight CDCA8, MAD2L1, and FANCI as potential targets for improving ES diagnosis and treatment.
Area of Science:
- Oncology
- Molecular Biology
- Genomics
Background:
- Ewing sarcoma (ES) is a prevalent pediatric bone cancer with poorly understood molecular mechanisms.
- Ethnic variations influence ES incidence and treatment outcomes, necessitating further research into its causes.
Purpose of the Study:
- To identify differentially expressed genes (DEGs) in ES.
- To elucidate the molecular mechanisms underlying ES.
- To discover potential diagnostic and therapeutic targets for ES.
Main Methods:
- Downloaded and analyzed three Gene Expression Omnibus (GEO) microarray datasets (GSE68776, GSE45544, GSE17674).
- Screened for DEGs and performed enrichment analysis.
- Constructed a protein-protein interaction (PPI) network using STRING and Cytoscape.
- Conducted survival and immune infiltration analyses.
Main Results:
- Identified 629 DEGs (206 up-regulated, 423 down-regulated).
- Enrichment analysis revealed involvement in pathways like cell cycle, p53, and cancer pathways.
- Screened 10 hub genes, with CDCA8, MAD2L1, and FANCI showing potential prognostic significance.
Conclusions:
- CDCA8, MAD2L1, and FANCI are implicated in the occurrence and prognosis of ES.
- This research provides insights into ES molecular mechanisms.
- Identified DEGs and key genes offer potential targets for ES diagnosis and treatment.
