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Screening of Potential Key Biomarkers for Ewing Sarcoma: Evidence from Gene Array Analysis
Duming Zhong1, Dan Chen1, Guangquan Zhang1
1Department of Orthopedics, The Fourth Affiliated Hospital of Nanchang University, Nanchang, People's Republic of China.
International Journal of General Medicine
|March 28, 2022
Summary
This study identifies key genes involved in Ewing sarcoma (ES), a childhood bone cancer. Findings highlight CDCA8, MAD2L1, and FANCI as potential targets for improving ES diagnosis and treatment.
Area of Science:
- Oncology
- Molecular Biology
- Genomics
Background:
- Ewing sarcoma (ES) is a prevalent pediatric bone cancer with poorly understood molecular mechanisms.
- Ethnic variations influence ES incidence and treatment outcomes, necessitating further research into its causes.
Purpose of the Study:
- To identify differentially expressed genes (DEGs) in ES.
- To elucidate the molecular mechanisms underlying ES.
- To discover potential diagnostic and therapeutic targets for ES.
Main Methods:
- Downloaded and analyzed three Gene Expression Omnibus (GEO) microarray datasets (GSE68776, GSE45544, GSE17674).
- Screened for DEGs and performed enrichment analysis.
- Constructed a protein-protein interaction (PPI) network using STRING and Cytoscape.
- Conducted survival and immune infiltration analyses.
Main Results:
- Identified 629 DEGs (206 up-regulated, 423 down-regulated).
- Enrichment analysis revealed involvement in pathways like cell cycle, p53, and cancer pathways.
- Screened 10 hub genes, with CDCA8, MAD2L1, and FANCI showing potential prognostic significance.
Conclusions:
- CDCA8, MAD2L1, and FANCI are implicated in the occurrence and prognosis of ES.
- This research provides insights into ES molecular mechanisms.
- Identified DEGs and key genes offer potential targets for ES diagnosis and treatment.
