Related Experiment Video

Updated: Sep 28, 2025

A Syngeneic Orthotopic Osteosarcoma Sprague Dawley Rat Model with Amputation to Control Metastasis Rate
07:31

A Syngeneic Orthotopic Osteosarcoma Sprague Dawley Rat Model with Amputation to Control Metastasis Rate

Published on: May 3, 2021

3.8K

GABPB1-AS1 Promotes the Development of Osteosarcoma by Targeting SP1 and Activating the Wnt/β-Catenin Pathway

Jingyang Chen1, Meiru Bian2,3, Lingxiao Pan1

  • 1Department of Orthopedics, Ningbo Medical Center Lihuili Hospital, Ningbo 315400, China.

Journal of Oncology
|March 28, 2022
PubMed

Insights

Long non-coding RNA GABPB1-AS1 promotes osteosarcoma (OS) growth by activating the SP1/Wnt/β-catenin pathway and inhibiting miR-199a-3p. Silencing GABPB1-AS1 reduces OS cell proliferation and migration.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Osteosarcoma (OS) is a primary bone malignancy with limited treatment options.
  • The role of long non-coding RNAs (lncRNAs) in OS pathogenesis is increasingly recognized.
  • GABPB1-AS1 has been implicated in various cancers, but its specific function in OS requires elucidation.

Purpose of the Study:

  • To investigate the role of GABPB1-AS1 in osteosarcoma (OS) progression.
  • To explore the underlying molecular mechanisms, including interactions with miR-199a-3p and the SP1/Wnt/β-catenin signaling pathway.

Main Methods:

  • GABPB1-AS1 expression was analyzed in OS cell lines (U2OS, HOS, MG63) and a normal cell line (hFOB1.19).
  • GABPB1-AS1 was silenced using siRNA in U2OS and HOS cells.
  • Cell proliferation, clonal formation, and migration were assessed using CCK-8, plate cloning, and Transwell assays.
  • Protein levels of SP1, Wnt, β-catenin, c-Myc, and SOX2 were analyzed via Western blot.
  • The interaction between GABPB1-AS1 and miR-199a-3p was confirmed using a dual-luciferase reporter assay.

Main Results:

  • GABPB1-AS1 expression was significantly upregulated in OS cell lines compared to hFOB1.19.
  • Silencing GABPB1-AS1 markedly inhibited proliferation, clonal formation, migration, and epithelial-mesenchymal transition in OS cells.
  • A direct binding relationship between GABPB1-AS1 and miR-199a-3p was established.
  • GABPB1-AS1 was found to upregulate SP1, Wnt, β-catenin, c-Myc, and SOX2, indicating activation of the SP1/Wnt/β-catenin pathway.

Conclusions:

  • GABPB1-AS1 acts as a carcinogenic factor in osteosarcoma.
  • GABPB1-AS1 promotes OS progression by competitively binding and inhibiting miR-199a-3p, thereby activating the SP1/Wnt/β-catenin signaling pathway.
  • GABPB1-AS1 represents a potential therapeutic target for osteosarcoma treatment.

More Related Videos

Intratibial Osteosarcoma Cell Injection to Generate Orthotopic Osteosarcoma and Lung Metastasis Mouse Models04:25

Intratibial Osteosarcoma Cell Injection to Generate Orthotopic Osteosarcoma and Lung Metastasis Mouse Models

Published on: October 28, 2021

10.0K
The Soft Agar Colony Formation Assay08:01

The Soft Agar Colony Formation Assay

Published on: October 27, 2014

112.4K

Related Concept Videos

TGF - β Signaling Pathway01:16

TGF - β Signaling Pathway

The TGF-β signaling pathway regulates cell growth, differentiation, adhesion, motility, and development. TGF-β ligands that induce TGF-β signaling are synthesized in their latent form. Several proteases or cell surface receptors such as integrins act upon the latent form, releasing the active ligand. There are three types of mammalian TGF-βs: (TGF-β1, TGF-β2, and TGF-β3) that bind as homodimers or heterodimers to TGF-β receptors. The TGF-β receptors...
7.8K
Canonical Wnt Signaling Pathway02:54

Canonical Wnt Signaling Pathway

The gene encoding the main signaling molecules of the Wnt signaling pathways (the Wnt proteins) was discovered almost four decades ago by Nüsslein-Volhard and Wieschaus. They identified and originally named the gene "wingless" (wg) after a phenotype discovered during their landmark genetic screen in Drosophila for body pattern defects. At around the same time, another researcher named Harold Varmus found that a murine tumor virus activates the mammalian wg homolog, Int-1, which...
9.1K
The JAK-STAT Signaling Pathway01:20

The JAK-STAT Signaling Pathway

Several cytokine receptors have tightly bound Janus kinase or JAK proteins attached at their cytosolic tail. Small signaling molecules such as cytokines, growth hormones, or prolactins bind to the cytokine receptors and initiate their dimerization. The dimerization brings the cytosolic JAKs together that trans-phosphorylate and activates each other. The activated JAKs now phosphorylate cytosolic tails of the cytokine receptors, which serve as binding sites for adaptor proteins such as  SH2...
9.4K
Non-Canonical Wnt Signaling Pathways01:41

Non-Canonical Wnt Signaling Pathways

Wnt is a zygotic effect gene that is expressed during very early embryonic development. It regulates various processes in animals starting from early development through the adult stage, such as organogenesis in the embryo and maintenance of neuronal and blood stem cells. Wnt proteins can induce a wide variety of intracellular pathways depending upon the specific abilities of different Wnt ligands to form a complex with shared and cognate receptors in the presence of different co-receptors. The...
7.5K
mTOR Signaling and Cancer Progression03:03

mTOR Signaling and Cancer Progression

The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
3.9K
Hedgehog Signaling Pathway02:33

Hedgehog Signaling Pathway

The Hedgehog gene (Hh) was first discovered due to its control of the growth of disorganized, hair-like bristles phenotype in Drosophila, much like hedgehog spines. Hh plays a crucial role in the development of organs and the maintenance of homeostasis in both invertebrates and vertebrates. However, while Drosophila has only one Hh protein, mammals have multiple functional Hedgehog proteins - Sonic (Shh), Desert (Dhh), and Indian Hedgehog (Ihh). All of these homologous proteins have adapted to...
7.6K