Related Experiment Video

Updated: Sep 28, 2025

Evaluation of Caspase Activation to Assess Innate Immune Cell Death
10:23

Evaluation of Caspase Activation to Assess Innate Immune Cell Death

Published on: January 20, 2023

3.4K

PIDD1 in cell cycle control, sterile inflammation and cell death

Elias S Weiler1, Tamas G Szabo1, Irmina Garcia-Carpio1

  • 1Institute for Developmental Immunology, Biocenter, Medical University of Innsbruck, Innsbruck, Austria.

Biochemical Society Transactions
|March 28, 2022
PubMed

Insights

Death domain protein PIDD1 regulates Caspase-2 activation and cell death. Its unique intein-like features enable diverse functions, highlighting its potential as a drug target for various diseases.

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Biochemistry

Background:

  • PIDD1 (death fold domain-containing protein) is a key regulator of Caspase-2, involved in cell death pathways.
  • Caspase-2 activation, crucial for cell cycle arrest due to centrosome aberrations, depends on the PIDDosome complex.
  • PIDD1 interacts with multiple proteins, influencing NF-κB activation, DNA synthesis, and cell death.

Purpose of the Study:

  • To review structural and molecular features of PIDD1.
  • To summarize recent advances in understanding PIDD1's diverse biological roles.
  • To highlight PIDD1's potential as a drug target.

Main Methods:

  • Literature review of structural and experimental data on PIDD1.
  • Analysis of PIDD1's intein-like self-cleaving properties.
  • Summary of studies on PIDD1's interactions and functions.

Main Results:

  • PIDD1 forms the core of the PIDDosome complex with RAIDD/CRADD for Caspase-2 activation.
  • PIDD1's intein-like mechanism allows generation of diverse polypeptides from a single precursor.
  • PIDD1 participates in NF-κB activation, DNA repair, and cell death pathways.

Conclusions:

  • PIDD1's unique self-cleaving mechanism underlies its diverse pathophysiological roles.
  • PIDD1 represents a promising drug target due to its involvement in multiple cellular processes.
  • Further research into PIDD1's structure-function relationships is warranted.
Keywords:
caspasescell cyclecell deathp53

More Related Videos

Cell Death Associated with Abnormal Mitosis Observed by Confocal Imaging in Live Cancer Cells15:53

Cell Death Associated with Abnormal Mitosis Observed by Confocal Imaging in Live Cancer Cells

Published on: August 21, 2013

15.1K
Generation of a RIP1 Knockout U937 Cell Line Using the CRISPR-Cas9 System08:15

Generation of a RIP1 Knockout U937 Cell Line Using the CRISPR-Cas9 System

Published on: April 11, 2025

465

Related Concept Videos

Negative Regulator Molecules01:23

Negative Regulator Molecules

Positive regulators allow a cell to advance through cell cycle checkpoints. Negative regulators have an equally important role as they terminate a cell’s progression through the cell cycle—or pause it—until the cell meets specific criteria.
36.3K
Inhibition of Cdk Activity02:34

Inhibition of Cdk Activity

The orderly progression of the cell cycle depends on the activation of Cdk protein by binding to its cyclin partner. However, the cell cycle must be restricted when undergoing abnormal changes. Most cancers correlate to the deregulated cell cycle, and since Cdks are a central component of the cell cycle, Cdk inhibitors are extensively studied to develop anticancer agents. For instance, cyclin D associates with several Cdks, such as Cdk 4/6, to form an active complex. The cyclin D-Cdk4/6 complex...
5.0K
DNA Damage can Stall the Cell Cycle02:37

DNA Damage can Stall the Cell Cycle

In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
9.4K
Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.7K
Molecular Factors Affecting Cell Division01:27

Molecular Factors Affecting Cell Division

Several external and internal factors influence the initiation and inhibition of cell division. For instance, the death of nearby cells or the release of human growth hormone (hGH) promotes cell division. In contrast, lack of hGH or crowding of cells can inhibit cell division.
Several proteins function as internal regulators to ensure each cell cycle stage is completed faithfully before proceeding to the next. Regulator molecules may act directly or influence the activity or production of other...
3.4K
The Cell Cycle Control System01:28

The Cell Cycle Control System

The cell cycle regulation directs how a cell proceeds from one phase to the next and begins mitosis. The cell cycle control system includes intracellular regulatory molecules and external triggers. They provide "stop" or "advance" signals and operate at specific cell cycle stages termed checkpoints to ensure that a particular process is completed before the cell advances to the next phase.
Cyclins and cyclin-dependent kinases (Cdks) are the primary cell cycle regulators and...
3.6K