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A Novel Approach toward Less Invasive Multiomics Gut Analyses: a Pilot Study
Adam J Berlinberg1, Ana Brar2, Andrew Stahly1
1Division of Rheumatology, Department of Medicine, University of Colorado, Anschutz Medical Campus, Aurora, Colorado, USA.
Microbiology Spectrum
|March 28, 2022
Summary
Cytology brushes offer a minimally invasive way to collect gut microbiome samples, showing good taxonomic diversity. However, biopsy samples are still recommended for accurate metabolomics assessment.
Area of Science:
- Gastroenterology
- Microbiology
- Biotechnology
Background:
- Gut microbiome and host interactions are crucial for health.
- Accurate sample collection is vital for 'omics' studies (metatranscriptomics, metabolomics).
- Current methods like biopsy are invasive, while fecal samples may not represent the host-microbe interface.
Purpose of the Study:
- To evaluate a novel, minimally invasive cytology brush method for human gut microbiome sampling.
- To compare metatranscriptomic and metabolomic data from cytology brush samples versus traditional intestinal biopsy samples.
Main Methods:
- Pilot study on 5 healthy participants undergoing colonoscopy.
- Collection of gut samples using both mucosal cytology brushes and intestinal biopsy.
- Comparative analysis of metatranscriptomic and metabolomic data between the two methods.
Main Results:
- Cytology brush samples showed increased taxonomic evenness and beta diversity.
- Similar microbial community transcriptional profiles were observed between brush and biopsy samples.
- Significant differences in metabolomics data suggest differential metabolite origins (bacterial vs. host-absorbed).
Conclusions:
- Cytology brushes are a valuable, less invasive tool for gut microbiome sampling.
- Metabolomics assessment requires careful consideration, as brush samples reflect metabolite production, not host absorption.
- Biopsy samples may remain necessary for comprehensive metabolomic analysis.
