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Complete Genome Sequence of Enterococcus faecalis Siphophage Sigurd
Adam Tomaszewski1, Daniel Mora2, James Clark1,2
1Department of Biochemistry and Biophysics, Texas A&M University, College Station, Texas, USA.
Microbiology Resource Announcements
|March 28, 2022
Summary
This study characterizes the Enterococcus faecalis siphophage Sigurd, revealing its genome structure and a terminase enzyme similar to Bacillus subtilis phage phi105. This research aids in understanding antibiotic-resistant bacterial phages.
Area of Science:
- Microbiology
- Virology
- Genomics
Background:
- Enterococcus faecalis is a significant cause of antibiotic-resistant infections.
- Bacteriophages (phages) are viruses that infect bacteria and are potential tools against bacterial pathogens.
Purpose of the Study:
- To present the genome sequence and characteristics of the E. faecalis siphophage, named Sigurd.
- To analyze the genomic structure and identify key genetic elements of Sigurd.
Main Methods:
- Genome sequencing of siphophage Sigurd.
- Bioinformatic analysis of the Sigurd genome.
- Comparative genomics to identify related phages.
Main Results:
- The complete genome of siphophage Sigurd is 41,811 base pairs.
- The genome is organized into two arms separated by a bidirectional terminator.
- Sigurd possesses a small terminase closely related to that of Bacillus subtilis phage phi105.
Conclusions:
- Siphophage Sigurd represents a novel phage with a distinct genomic organization.
- The identified terminase provides insights into phage evolution and classification.
- Understanding Sigurd can contribute to phage therapy strategies against E. faecalis infections.
