Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Crystal Growth: Principles of Crystallization01:25

Crystal Growth: Principles of Crystallization

2.9K
Crystallization is a phase transformation process in which crystals are precipitated from a supersaturated solution or formed from other sources. During crystallization, atoms or molecules arrange themselves into a well-defined, rigid crystal lattice to minimize energy.
Initiating crystallization involves manipulating the concentration of the solute and the temperature of the solution. Since crystal growth occurs when the ratio of concentration and solubility of the solute in the solvent...
2.9K
X-ray Crystallography02:18

X-ray Crystallography

24.4K
The size of the unit cell and the arrangement of atoms in a crystal may be determined from measurements of the diffraction of X-rays by the crystal, termed X-ray crystallography.
Diffraction
Diffraction is the change in the direction of travel experienced by an electromagnetic wave when it encounters a physical barrier whose dimensions are comparable to those of the wavelength of the light. X-rays are electromagnetic radiation with wavelengths about as long as the distance between neighboring...
24.4K
Recrystallization: Solid–Solution Equilibria01:10

Recrystallization: Solid–Solution Equilibria

1.2K
Recrystallization is a purification technique used to separate impurities from solid compounds. In this technique, no chemical reactions occur. Instead, it exploits physical properties only, specifically, the solubility differences between the desired compound and impurities, either at a single temperature or at different temperatures, and under other selected conditions. The solid-solution equilibrium (solubility equilibrium) of each component in the solution represents a binary phase...
1.2K
X-ray Diffraction of Biological Samples01:10

X-ray Diffraction of Biological Samples

4.1K
X-ray diffraction or XRD is an analytical tool that utilizes X-rays to study ordered structures such as crystalline organic and inorganic samples, polycrystalline materials, proteins, carbohydrates, and drugs.
According to Bragg's law, when X-rays strike the sample positioned on a stage, the rays are  scattered by the electron clouds around the sample atoms. The  X-ray diffraction or scattering is caused by constructive interference of the X-ray waves that reflect off the internal...
4.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Stationary state distribution and efficiency analysis of the Langevin equation via real or virtual dynamics.

The Journal of chemical physics·2017
Same author

Optimized production and isolation of antibacterial agent from marine <i>Aspergillus flavipes</i> against <i>Vibrio harveyi</i>.

3 Biotech·2017
Same author

Preparation and antioxidant properties of low molecular holothurian glycosaminoglycans by H<sub>2</sub>O<sub>2</sub>/ascorbic acid degradation.

International journal of biological macromolecules·2017
Same author

Associations between single-nucleotide polymorphisms in the NTRK1 gene and basal pain sensitivity in young Han Chinese women.

Neuroscience letters·2017
Same author

Design, synthesis and antiproliferative effect of 17β-amide derivatives of 2-methoxyestradiol and their studies on pharmacokinetics.

Steroids·2017
Same author

MiR-324-3p promotes tumor growth through targeting DACT1 and activation of Wnt/β-catenin pathway in hepatocellular carcinoma.

Oncotarget·2017

Related Experiment Video

Updated: Sep 28, 2025

On-Chip Crystallization and Large-Scale Serial Diffraction at Room Temperature
07:42

On-Chip Crystallization and Large-Scale Serial Diffraction at Room Temperature

Published on: March 11, 2022

2.0K

On-chip Crystallization and Large-Scale Serial Diffraction at Room Temperature.

Linta M Biju1, Cong Wang1, Weijia Kang1

  • 1Department of Chemistry, University of Illinois at Chicago.

Journal of Visualized Experiments : Jove
|March 28, 2022
PubMed
Summary

This study introduces a novel crystal-on-crystal device for in situ X-ray diffraction at room temperature. This method bypasses cryo-cooling, enabling natural protein dynamics observation without sample manipulation for high-throughput screening.

More Related Videos

Microfluidic Chips for In Situ Crystal X-ray Diffraction and In Situ Dynamic Light Scattering for Serial Crystallography
11:48

Microfluidic Chips for In Situ Crystal X-ray Diffraction and In Situ Dynamic Light Scattering for Serial Crystallography

Published on: April 24, 2018

14.9K
Crystallization and Structural Determination of an Enzyme:Substrate Complex by Serial Crystallography in a Versatile Microfluidic Chip
10:45

Crystallization and Structural Determination of an Enzyme:Substrate Complex by Serial Crystallography in a Versatile Microfluidic Chip

Published on: March 20, 2021

8.5K

Related Experiment Videos

Last Updated: Sep 28, 2025

On-Chip Crystallization and Large-Scale Serial Diffraction at Room Temperature
07:42

On-Chip Crystallization and Large-Scale Serial Diffraction at Room Temperature

Published on: March 11, 2022

2.0K
Microfluidic Chips for In Situ Crystal X-ray Diffraction and In Situ Dynamic Light Scattering for Serial Crystallography
11:48

Microfluidic Chips for In Situ Crystal X-ray Diffraction and In Situ Dynamic Light Scattering for Serial Crystallography

Published on: April 24, 2018

14.9K
Crystallization and Structural Determination of an Enzyme:Substrate Complex by Serial Crystallography in a Versatile Microfluidic Chip
10:45

Crystallization and Structural Determination of an Enzyme:Substrate Complex by Serial Crystallography in a Versatile Microfluidic Chip

Published on: March 20, 2021

8.5K

Area of Science:

  • Structural biology
  • Biophysics
  • Crystallography

Background:

  • Understanding protein functional states requires observing their dynamic transitions.
  • Cryogenic temperatures used in traditional X-ray diffraction are non-physiological and can alter protein dynamics.
  • A method for routine X-ray diffraction at room temperature is needed to study proteins in their native-like states.

Purpose of the Study:

  • To present a protocol for a novel crystal-on-crystal device enabling in situ X-ray diffraction at room temperature.
  • To demonstrate a high-throughput method for analyzing protein crystals without sample manipulation.
  • To facilitate the study of protein structural dynamics under physiological conditions.

Main Methods:

  • Development and assembly of the crystal-on-crystal device with integrated hardware and software.
  • On-chip crystallization of protein samples.
  • Optical scanning and automated crystal recognition for X-ray shot planning.
  • Automated data collection using X-ray diffraction at room temperature.

Main Results:

  • The protocol details key steps from device assembly to automated data collection.
  • The crystal-on-crystal platform allows in situ X-ray diffraction at room temperature for various crystal sizes.
  • Hundreds to thousands of protein crystals can be analyzed in a programmable, high-throughput manner without harvesting or manipulation.

Conclusions:

  • The presented protocol offers a robust method for routine X-ray diffraction experiments at room temperature.
  • This approach overcomes limitations of cryogenic methods, allowing for the study of native protein structural dynamics.
  • The high-throughput capability of the crystal-on-crystal device significantly advances structural biology research.