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Related Concept Videos

Chirality in Nature02:30

Chirality in Nature

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Chirality is the most intriguing yet essential facet of nature, governing life’s biochemical processes and precision. It can be observed from a snail shell pattern in a macroscopic world to an amino acid, the minutest building block of life. Most of the snails around the world have right-coiled shells because of the intrinsic chirality in their genes. All the amino acids present in the human body exist in an enantiomerically pure state, except for glycine - the sole achiral amino acid.
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Related Experiment Video

Updated: Sep 28, 2025

A Micropatterning Assay for Measuring Cell Chirality
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A Micropatterning Assay for Measuring Cell Chirality

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A Micropatterning Assay for Measuring Cell Chirality.

Haokang Zhang1, Kacey Ronaldson-Bouchard2, Gordana Vunjak-Novakovic3

  • 1Department of Biomedical Engineering, Rensselaer Polytechnic Institute; Center for Biotechnology & Interdisciplinary Studies, Rensselaer Polytechnic Institute.

Journal of Visualized Experiments : Jove
|March 28, 2022
PubMed
Summary

This study presents a new method to measure cell chirality, a key cellular property. The assay uses micropatterned cells to reliably quantify directional cell migration, advancing research in development and disease.

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Last Updated: Sep 28, 2025

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Area of Science:

  • Cell Biology
  • Biophysics
  • Biotechnology

Background:

  • Chirality, or cellular asymmetry, is crucial in biological development and disease.
  • Standardized methods for quantifying cell chirality are needed to advance research.
  • Existing methods lack the precision required for detailed chiral analysis.

Purpose of the Study:

  • To develop and validate a reliable protocol for characterizing multicellular chirality.
  • To establish a standardized assay for quantifying directional cell migration.
  • To provide a tool for investigating the roles of cell chirality in biological processes.

Main Methods:

  • Fabrication of micropatterned substrates using microcontact printing on coated glass slides.
  • Seeding cells on geometrically defined, protein-coated islands for directed migration.
  • Automated analysis and quantification of cell alignment using a custom MATLAB program.

Main Results:

  • Demonstrated successful fabrication of micropatterned cell arrays.
  • Showcased directional cell migration with biased alignment (clockwise or counterclockwise).
  • Validated the protocol's efficiency and reliability with NIH/3T3 cells.

Conclusions:

  • The described multicellular chirality characterization assay is an efficient and reliable in vitro tool.
  • This protocol advances the study of cell chirality and its implications in development and disease.
  • Standardized quantification of cell chirality facilitates further research and potential applications.