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Tissue Homogenization and Cell Lysis01:32

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Tissue homogenization involves disintegrating tissue architecture and lysing cells, and is an early step in isolating and analyzing cellular components. The method used for homogenization depends on the sample type, the amount of sample available, the analyte to be obtained, and the sensitivity of the method. These methods are broadly classified as mechanical and non-mechanical methods.
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Automated variable power cold microwave tissue processing: A novel universal tissue processing protocol without using

Naser Haghbin1, Behrouz Oveisi2, Amir Parsa Banitaba3

  • 1Department of Mechanical Engineering, Fairfield University, 1073 North Benson Rd., Fairfield, CT 06824, USA.

Acta Histochemica
|March 28, 2022
PubMed
Summary

A new automated microwave tissue processor uses a universal protocol to process diverse tissue types up to 4mm thick in 97 minutes. This method improves safety by reducing hazardous reagents and ensures high-quality tissue preservation for diagnosis.

Keywords:
Fatty and nonfatty tissues processingRapid tissue processingVariable power cold microwave tissue processing (VCT)

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Area of Science:

  • Histopathology
  • Biomedical Engineering
  • Laboratory Science

Background:

  • Traditional tissue processing is time-sensitive and prone to errors, especially with varied tissue types and sizes.
  • Standard protocols require different batches for different tissues, increasing complexity and potential for human error.
  • Concerns exist regarding the health and environmental impacts of hazardous reagents like formaldehyde and xylene used in conventional methods.

Purpose of the Study:

  • To develop and evaluate a novel automated variable power cold microwave tissue processor.
  • To establish a universal tissue processing protocol for simultaneous processing of diverse tissue types.
  • To assess the safety and efficacy of the new processor using environmentally friendlier reagents.

Main Methods:

  • Development of an automated variable power cold microwave tissue processor.
  • Implementation of a universal 97-minute processing protocol for tissues up to 4mm thick.
  • Evaluation of processed tissues (fatty and non-fatty) using MAS (morphology, artifacts, staining) criteria under light microscopy.

Main Results:

  • The novel processor successfully processed various healthy and diseased tissues, including fatty samples.
  • All processed tissue samples met the defined MAS criteria for quality assessment.
  • Microscopic examination revealed outstanding tissue integrity, arrangement, and visual distinction without formaldehyde or xylene.

Conclusions:

  • The automated microwave tissue processor offers a safe, efficient, and universal solution for tissue processing.
  • This technology minimizes hazardous reagent use and human error, enhancing diagnostic capabilities.
  • The processed tissues exhibit excellent quality suitable for detailed histopathological analysis.