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IncFIB-4.1 and IncFIB-4.2 Single-Replicon Plasmids: Small Backbones with Large Accessory Regions
Yanan Xu1, Ying Jing2, Lingfei Hu2
1Department of Clinical Laboratory Medicine, Hebei Medical University, Shijiazhuang, Hebei, 050011, People's Republic of China.
Infection and Drug Resistance
|March 29, 2022
Summary
A new typing scheme for IncFIB replicons was developed, revealing IncFIB-4.1/4.2 plasmids carry diverse mobile genetic elements and antibiotic resistance genes, highlighting their role in drug resistance evolution.
Area of Science:
- Microbiology
- Genomics
- Plasmid Biology
Background:
- IncFIB replicons are crucial genetic elements found in various bacteria.
- Understanding their diversity and genomic features is essential for tracking antimicrobial resistance.
Purpose of the Study:
- To establish a robust typing scheme for IncFIB replicons.
- To analyze the genomic characteristics of IncFIB-4.1/4.2 single-replicon plasmids.
Main Methods:
- Phylogenetic analysis of 146 repBIncFIB sequences to create a typing scheme.
- Whole-genome sequencing and comparative analysis of nine IncFIB-4.1/4.2 single-replicon plasmids from China and existing GenBank data.
Main Results:
- A repB sequence-based scheme classified IncFIB replicons into seven primary types and 70 subtypes.
- Eleven IncFIB-4.1/4.2 single-replicon plasmids shared common backbone markers but exhibited distinct large accessory regions (LARs) with varied mobile genetic elements (MGEs) and gene loci.
- Antibiotic resistance regions (ARRs) were present in seven LARs, containing transposons, integrons, and novel MGEs (In1776, Tn6755, Tn6857).
Conclusions:
- IncFIB-4.1/4.2 single-replicon plasmids are significant vectors for drug resistance genes.
- The study provides insights into the diversity and evolution of IncFIB replicons and associated plasmids.
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