Carbon quantum dots with blue/near infrared emissions for ratiometric fluorescent lornoxicam sensing and bio-imaging

An economical and eco-friendly hydrothermal method for the preparation of nitrogen-doped carbon quantum dots (N-CQDs) was studied with rambutan peel and lysine. The morphology, structure, and optical properties of N-CQDs were characterized by transmission electron microscopy, Fourier transform infrared spectrometry, X-ray powder diffractometer, X-ray photoelectron spectrometry, and UV spectrophotometry. The synthesized N-CQDs have excellent characteristics such as strong fluorescence, good dispersion, high stability, and excellent water solubility. The absolute fluorescence quantum yield is 1.02%, the average particle size is 1.63 nm, and the maximum excitation wavelength is 340 nm. The maximum emission wavelengths are 430 nm and 800 nm. As a quencher, lornoxicam (LNX) was used to quench the fluorescence of N-CQDs with the mechanism of inner filter effect. The fluorescence ratio of N-CQDs (F₄₃₀/F₈₀₀) shows a good linear relationship to the concentration of LNX. The linear range and the detection limit of LNX are 0.01‒100 and 0.003 μmol/L, respectively. An effective ratiometric fluorescence probe for the detection of LNX was constructed. The method has the advantages of low detection limit, high sensitivity, wide linear range, and can be applied to the determination of LNX in real samples. Moreover, according to the excitation-dependent fluorescence behavior, dual-wavelength emission, and biocompatibility of N-CQDs, it has been applied to cell imaging.