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Improving selenium accumulation in broilers using Escherichia coli Nissle 1917 with surface-displayed selenite
Yuxin Gong1, Ying Wu1, Aman Khan1
1Gansu Key Laboratory of Biomonitoring and Bioremediation for Environmental Pollution, School of Life Sciences, Lanzhou University, Tianshuinanlu #222, Lanzhou 730000, Gansu, China. xkli@lzu.edu.cn.
Food & Function
|March 29, 2022
Summary
This study engineered bacteria to deliver selenium nanoparticles (SeNPs) to broilers, enhancing selenium levels and antioxidant enzymes. This novel approach improves selenium utilization and broiler health.
Area of Science:
- Biotechnology
- Animal Nutrition
- Microbiology
Background:
- Selenium is vital for livestock and poultry health.
- Selenium nanoparticles (SeNPs) are efficient supplements.
- A high-efficiency selenite reductase (SerV01) was identified in Staphylococcus aureus LZ-01.
Purpose of the Study:
- To engineer a probiotic bacterium for enhanced selenium delivery in broilers.
- To evaluate the efficacy of surface display-engineered E. coli Nissle 1917 (EcN) carrying SerV01 for selenium supplementation.
- To assess the impact on broiler selenium content, antioxidant status, and gut microbiota.
Main Methods:
- Identified and isolated the SerV01 enzyme from Staphylococcus aureus LZ-01.
- Engineered E. coli Nissle 1917 (EcN) to display SerV01 on its surface (EcN-IS).
- Administered EcN-IS to broilers and measured selenium levels, antioxidant enzyme activity, and gut flora composition.
Main Results:
- EcN-IS significantly increased selenium content in liver (0.87 mg kg-1), breast muscle (0.52 mg kg-1), and serum (6.10 mg L-1).
- Glutathione peroxidase and thioredoxin reductase levels were elevated with SeNPs + EcN-IS treatment.
- Beneficial intestinal flora, including Lactobacillus and Propionibacterium, increased significantly.
Conclusions:
- Surface display-engineered E. coli Nissle 1917 effectively delivers selenium nanoparticles in broilers.
- This technology enhances selenium bioavailability and antioxidant capacity in poultry.
- The approach offers a novel strategy for optimizing selenium utilization in broiler diets.
