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Updated: Sep 28, 2025

Probing RNA Structure with Dimethyl Sulfate Mutational Profiling with Sequencing In Vitro and in Cells
Published on: December 9, 2022
Probing RNA structures and functions by solvent accessibility: an overview from experimental and computational
Md Solayman1, Thomas Litfin1, Jaswinder Singh2
1Institute for Glycomics, Griffith University, Parklands Dr. Southport, QLD 4222, Australia.
Abstract:
Characterizing RNA structures and functions have mostly been focused on 2D, secondary and 3D, tertiary structures. Recent advances in experimental and computational techniques for probing or predicting RNA solvent accessibility make this 1D representation of tertiary structures an increasingly attractive feature to explore. Here, we provide a survey of these recent developments, which indicate the emergence of solvent accessibility as a simple 1D property, adding to secondary and tertiary structures for investigating complex structure-function relations of RNAs.
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