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Partial purification and characterization of a thermostable xylanase from Bacillus licheniformis isolated from hot
Girisha Malhotra1, Shilpa S Chapadgaonkar2
1Manav Rachna International Institute of Research and Studies, Faridabad, Haryana, India.
Background:
Thermo-alkali stable xylanases were purified from the extracellular broth of newly isolated Bacillus licheniformis strain produced in a 5-L stirred-tank bioreactor with wheat bran as a carbon source.
Results:
A high degree of purity was achieved using size exclusion chromatography resulting in 16-fold purification and 69% recovery for fraction 5 which had the highest activity. The recovery obtained after pooling fractions 5 and 6 was 99%. The Km value of xylanase was calculated as 0.05 mM, and Vmax was 125 μmol/min/mg protein.
Conclusion:
Purified xylanase had a high thermal and pH stability. Xylanase was found to be suitable for application in the de-inking of paper and for saccharification of lignocellulosic waste biomass.
