Polycyclic aromatic hydrocarbons, long non-coding RNA expression, and DNA damage in coke oven workers
1Department of Occupational and Environmental Health, Key Laboratory of Environment and Health, Ministry of Education & Ministry of Environmental Protection, State Key Laboratory of Environmental Health (Incubating), School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, 13 Hangkong Rd., Wuhan, 430030, Hubei, China.
Long non-coding RNA NR_024564 may influence DNA damage in coke oven workers with over 20 years of exposure to polycyclic aromatic hydrocarbons (PAHs). Lower NR_024564 levels correlated with increased DNA damage markers, especially with higher PAH metabolite exposure.
Area of Science:
- Environmental Health
- Molecular Biology
- Occupational Medicine
Background:
- Polycyclic Aromatic Hydrocarbons (PAHs) exposure is linked to DNA damage.
- The role of long non-coding RNAs (lncRNAs) in this association is not well understood.
- Coke oven workers face significant PAH exposure.
Purpose of the Study:
- To investigate the association between lncRNA NR_024564, urinary monohydroxy PAHs (OH-PAHs), and DNA damage.
- To explore these relationships in coke oven workers with varying years of service.
Main Methods:
- Quantified 12 OH-PAHs using gas chromatography-mass spectrometry.
- Measured NR_024564 expression via droplet digital RT-PCR.
- Assessed DNA damage using the comet assay in 332 coke oven workers.
Main Results:
- NR_024564 was not associated with OH-PAHs or DNA damage in the total cohort.
- In workers with ≥20 years of service, multiple OH-PAHs correlated with increased DNA damage.
- NR_024564 was associated with 2-OHPh and DNA damage markers; lower NR_024564 correlated with higher DNA damage.
Conclusions:
- NR_024564 may play a role in the DNA damage associated with PAH exposure in long-term coke oven workers.
- The findings suggest NR_024564 as a potential biomarker or mediator in occupational PAH exposure and DNA damage.
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