Serial Block Electron Microscopy to Study Plasmodesmata in the Vasculature of Arabidopsis thaliana Roots

Serial block electron microscopy (SB-EM) is a technique that enables acquisition and reconstruction of 3D cellular volumes. The approach is valuable for the study of plasmodesmata (PD) as the relative positions of these structures are contained in the datasets. In this chapter, we describe how to prepare plant roots for SB-EM via fixation, embedding, and trimming steps. We also provide details and recommendations for later image acquisition and processing. The procedure is suitable to work on root vascular tissues.