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Related Experiment Video

Updated: Sep 28, 2025

Laboratory Estimation of Net Trophic Transfer Efficiencies of PCB Congeners to Lake Trout Salvelinus namaycush from Its Prey

Laboratory Estimation of Net Trophic Transfer Efficiencies of PCB Congeners to Lake Trout Salvelinus namaycush from Its Prey

Published on: August 29, 2014

Interlaboratory Comparison of Three Sediment Bioaccumulation Tests.

Guilherme R Lotufo¹, James M Biedenbach¹, J Daniel Farrar¹

¹US Army Engineer Research and Development Center, Vicksburg, Mississippi, USA.

Environmental Toxicology and Chemistry

|March 29, 2022

Summary

This summary is machine-generated.

Interlaboratory variability in standard bioaccumulation tests using clams and worms was assessed. Results show low variability, but the biological significance of findings should consider the magnitude of differences.

Keywords:

Alitta virens Benthic macroinvertebrates Bioaccumulation Lumbriculus variegatus Macoma nasuta Polychlorinated biphenyls Sediment assessment

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Physical, Chemical and Biological Characterization of Six Biochars Produced for the Remediation of Contaminated Sites

Published on: November 28, 2014

Area of Science:

Environmental Toxicology
Ecotoxicology
Bioaccumulation Studies

Background:

Standard bioaccumulation tests utilize Macoma nasuta (clam), Alitta virens (polychaete), and Lumbriculus variegatus (oligochaete).
Interlaboratory variability in these standard tests was previously unknown, impacting data interpretation.

Purpose of the Study:

To quantify interlaboratory and intralaboratory variability in standard 28-day bioaccumulation tests.
To assess the impact of lipid normalization on variability for polychlorinated biphenyls (PCBs) and polycyclic aromatic hydrocarbons (PAHs).
To evaluate analytical variability in tissue sample analysis.

Main Methods:

Four laboratories conducted standard 28-day bioaccumulation tests with M. nasuta, A. virens, and L. variegatus using PCB- and PAH-contaminated sediments.
A single laboratory performed chemical analysis of tissue samples.
Analytical variability was assessed by multiple laboratories analyzing tissue homogenates for PCBs and lipids.

Main Results:

Intralaboratory variance for PCB tissue concentrations was generally low (CVs 9-28%), except for one lab with L. variegatus (CV 51%).
Intralaboratory variance exceeded interlaboratory variance for A. virens and L. variegatus (MOD 1.4-2.0).
Lipid normalization had minimal impact on variability; analytical variability was higher than bioassay variability only for L. variegatus.

Conclusions:

Low intralaboratory variability was observed, but the magnitude of difference (MOD) suggests limited biological significance for statistically significant findings.
Considering MOD is crucial for interpreting bioaccumulation data and accounting for inherent test variability.
Standardized bioaccumulation tests show manageable variability, but careful interpretation considering effect size is recommended.