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Updated: Sep 28, 2025

Microbiota Analysis Using Two-step PCR and Next-generation 16S rRNA Gene Sequencing
Published on: October 15, 2019
Gavin A Kuziel1, Seth Rakoff-Nahoum1
1Division of Infectious Diseases and Division of Gastroenterology, Department of Pediatrics, Boston Children's Hospital, Department of Microbiology, Harvard Medical School, Boston, MA 02115, USA.
The gut microbiome, comprising diverse microbes in animal intestines, influences host health. This review explores how hosts and environments shape these microbial communities and their impact on mammalian physiology.
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