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Microfluidic paper-based aptasensor devices for multiplexed detection of pathogenic bacteria.

Sandeep B Somvanshi¹, Ana M Ulloa², Min Zhao³

¹School of Materials Engineering, Purdue University, West Lafayette, IN, 47907, United States; Department of Physics, Dr. Babasaheb Ambedkar Marathwada University, Aurangabad, 431004, Maharashtra, India.

Biosensors & Bioelectronics

|March 29, 2022

Summary

This summary is machine-generated.

This study presents a novel paper-based microfluidic device for simultaneous detection of foodborne pathogens like E. coli O157:H7 and S. Typhimurium. The device offers quantitative results through image analysis for rapid on-site screening.

Keywords:

Aptasensors Colorimetric sensors Multiplexed detection Pathogenic bacteria

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Area of Science:

Biotechnology
Analytical Chemistry
Public Health

Background:

Foodborne pathogens pose significant global health risks.
Paper-based microfluidic devices offer a low-cost, user-friendly platform for diagnostics.
Multiplexed detection of multiple pathogens is crucial for comprehensive food safety monitoring.

Purpose of the Study:

To develop a novel paper-based microfluidic device for simultaneous, quantitative detection of whole-cell foodborne bacteria.
To enable rapid, on-site screening of pathogenic bacteria in food and water samples.

Main Methods:

A single-input channel microfluidic paper-based device was designed.
Multiplexed aptasensors were developed for simultaneous detection of E. coli O157:H7 and S. Typhimurium.
Colorimetric signal enhancement and quantitative analysis via image processing were employed.

Main Results:

The device achieved simultaneous detection of E. coli O157:H7 and S. Typhimurium.
Quantitative results showed linearity from 10^2 to 10^8 CFU/mL.
Limits of detection were 10^3 CFU/mL for E. coli O157:H7 and 10^2 CFU/mL for S. Typhimurium.
High specificity was observed against non-target bacteria.

Conclusions:

The developed multiplexed aptasensor is a promising tool for rapid, on-site screening of foodborne pathogens.
This technology can significantly contribute to improving food safety and public health surveillance.