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Interaction of Human Dendritic Cells with Graphene Oxide Nanoparticles In Vitro
S V Uzhviyuk1, M S Bochkova2, V P Timganova2
1Institute of Ecology and Genetics of Microorganisms, Ural Division of the Russian Academy of Sciences - Affiliated Branch of Perm' Federal Research Center, Ural Division of the Russian Academy of Sciences, Perm, Russia. kochurova.sofja@yandex.ru.
Bulletin of Experimental Biology and Medicine
|March 30, 2022
Summary
Graphene oxide nanoparticles did not harm human dendritic cells but can suppress their maturation. High concentrations of P-GO nanoparticles reduced dendritic cell differentiation by inhibiting CD83 expression.
Area of Science:
- Nanotechnology
- Immunology
- Cell Biology
Background:
- Dendritic cells (DCs) are crucial for immune responses.
- Nanoparticles are increasingly used in biomedical applications.
- Understanding nanoparticle interactions with immune cells is vital.
Purpose of the Study:
- To investigate the impact of graphene oxide nanoparticles (GO NPs) on human dendritic cell differentiation and NP uptake.
- To assess the effects of GO NP size, PEGylation, and concentration on DCs.
Main Methods:
- Human peripheral blood mononuclear cells were differentiated into DCs using IL-6 and GM-CSF.
- Graphene oxide nanoparticles functionalized with linear or branched PEG (P-GO, bP-GO) were used.
- Cell viability, DC percentage, CD83 expression, and NP uptake were analyzed at varying NP concentrations (5 and 25 μg/ml).
Main Results:
- GO NPs did not affect DC viability or percentage.
- P-GO nanoparticles at 25 μg/ml suppressed CD83 expression, indicating inhibited DC differentiation.
- DCs internalized P-GO NPs, with higher concentrations leading to more active engulfment, irrespective of NP size or PEG type.
Conclusions:
- Graphene oxide nanoparticles, particularly P-GO at 25 μg/ml, can modulate dendritic cell differentiation by suppressing maturation.
- The findings suggest potential immunomodulatory effects of GO NPs, warranting further investigation for therapeutic applications.
