Protective Effect of Calcium Fructoborate Against Carbon Tetrachloride-Induced Toxicity in Rats

Area of Science:

• Toxicology
• Nutritional Biochemistry
• Pharmacology

Background:

• Carbon tetrachloride (CCl4) is a known hepatotoxin that induces oxidative stress and cellular damage.
• Boron-based nutritional supplements, like Calcium fructoborate (CFB), possess antioxidant properties.
• CFB's chemical structure resembles naturally occurring boron in edible plants.

Purpose of the Study:

• To evaluate the antioxidant activity of CFB.
• To assess the protective effects of CFB against CCl4-induced DNA damage.
• To determine the histopathological impact of CFB on liver and kidney tissues following CCl4 toxicity.

Main Methods:

• Administered CCl4 to induce toxicity in Wistar albino rats over 14 days.
• Treated groups received varying doses of CFB (FruiteX-B) or olive oil.
• Analyzed serum biochemical markers (AST, ALT, HDL, LDH, urea, creatinine, etc.).
• Assessed antioxidant enzymes (CAT, GR, GPx, SOD) and oxidative stress markers (GSH, MDA, 8-OHdG) in liver and kidney tissues.
• Conducted histopathological examinations of liver and kidney tissues.

Main Results:

• CCl4 significantly disrupted antioxidant defense mechanisms and enzyme systems in the liver and kidneys.
• CFB treatment demonstrated significant antioxidant activity, regulating antioxidant metabolism.
• CFB exhibited a protective effect against CCl4-induced DNA damage, as evidenced by reduced 8-OHdG levels.
• Histopathological analysis supported the protective role of CFB against CCl4-induced tissue damage.

Conclusions:

• CFB, a boron-based dietary supplement, possesses significant antioxidant properties.
• CFB effectively counteracts CCl4-induced oxidative stress and protects liver and kidney tissues.
• CFB demonstrates a protective effect against DNA damage and histopathological alterations caused by CCl4 toxicity.