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A Radiolabeling Method for Precise Quantification of Polymers.

Lina Liu1, Talena Rambarran1, Ben Muirhead1

  • 1Department of Chemical Engineering, McMaster University, 1280 Main St. West, Hamilton, Ontario L8S 4L7, Canada.

Bioconjugate Chemistry
|March 30, 2022
PubMed
Summary
This summary is machine-generated.

This study introduces a novel 125I radiolabeling method for precise polymer quantification. The technique accurately measured hyaluronic acid (HA) on contact lenses and demonstrated enhanced retention of phenylboronic acid (PBA) micelles in vivo.

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Area of Science:

  • Biochemistry and Biomaterials Science
  • Pharmacology and Drug Delivery
  • Radiochemistry and Polymer Science

Background:

  • Accurate quantification of polymers is crucial in various research fields.
  • Radiolabeling offers a precise method for tracking molecules.
  • Existing methods may lack sensitivity for microgram quantities.

Purpose of the Study:

  • To develop and validate a versatile 125I radiolabeling method for polymers.
  • To quantify hyaluronic acid (HA) uptake and release on contact lenses.
  • To evaluate the in vivo retention of mucoadhesive micelles using radiolabeling.

Main Methods:

  • Introduction of phenol groups onto hyaluronic acid (HA) and block copolymers.
  • 125I labeling of phenol-functionalized polymers using a modified ICl method.
  • Quantification of HA on contact lenses and in vivo distribution of micelles in rats.

Main Results:

  • 125I labeling enabled accurate quantification of microgram amounts of HA.
  • Phenylboronic acid (PBA) micelles showed approximately four times higher retention in nasopharyngeal tissues.
  • The radiolabeling method proved effective for diverse polymer quantification applications.

Conclusions:

  • The developed 125I radiolabeling technique is highly effective for precise polymer quantification.
  • This method facilitates the study of polymer interactions with biological surfaces and in vivo behavior.
  • The findings support the use of PBA-functionalized polymers for enhanced mucosal retention.