Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Protein Folding Quality Check in the RER01:29

Protein Folding Quality Check in the RER

4.0K
ER is the primary site for the maturation and folding of soluble and transmembrane secretory proteins. The calnexin cycle is a specific chaperone system that folds and assesses the confirmation of N-glycosylated proteins before they can exit the ER lumen. The primary players of this quality check pipeline are the lectins, ER-resident chaperones, and a glucosyl transferase enzyme. In case the calnexin system in the lumen fails to salvage a misfolded protein, it is transported to the cytoplasm...
4.0K
Peptidoglycan Synthesis01:28

Peptidoglycan Synthesis

569
Structure of PeptidoglycanPeptidoglycan is a vital structural component of the bacterial cell wall, providing mechanical strength and shape to the cell. It consists of repeating units of two sugars—N-acetylglucosamine (NAG) and N-acetylmuramic acid (NAM)—linked by β-1,4 glycosidic bonds. These sugar chains are cross-linked by short peptide chains, forming a mesh-like polymer that surrounds the bacterial plasma membrane.Cytoplasmic Phase – Precursor SynthesisPeptidoglycan...
569
Oligosaccharide Assembly01:24

Oligosaccharide Assembly

3.0K
Protein glycosylation starts in the ER lumen and continues in the Golgi apparatus. Glycosyltransferases catalyze the addition of sugar molecules or glycosylation of proteins. Usually, these enzymes add sugars to the hydroxyl groups of selected serine or threonine residues to form O-linked glycans or the amino groups of asparagine residues to form N-linked glycans. Different positions on the same polypeptide chain can contain differently linked glycans.
Multiple sugar molecules that may or may...
3.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Defective queuosine and i6A/ms2i6A modification of tRNATyr cause frameshifting and protein aggregation.

Nucleic acids research·2026
Same author

Seed Priming with Alginate-Whey Protein Concentrate Encapsulated Bacillus velezensis VRU1 Enhances Growth of Pistachio Seedlings.

Current microbiology·2026
Same author

Synthesis of N<i>-</i>alkylated hypoxanthine derivatives and their evaluation as antimicrobial agents.

Journal of environmental science and health. Part. B, Pesticides, food contaminants, and agricultural wastes·2026
Same author

Incidence and outcomes of patients hospitalised with COPD in Greater Glasgow and Clyde, Scotland 2015-2022: a retrospective cohort study.

BMC pulmonary medicine·2026
Same author

Safety and effectiveness of ETHIZIA versus TachoSil for hemostasis during open liver surgery: a randomized controlled multicenter trial.

HPB : the official journal of the International Hepato Pancreato Biliary Association·2026
Same author

Targeting traumatic brain injury via novel tetrahydrocarbazole derivatives: <i>in silico</i> design, synthesis, and <i>in vitro</i> evaluation of dual NADPH oxidase 2 and 4 inhibitors.

Future medicinal chemistry·2026

Related Experiment Video

Updated: Sep 28, 2025

Molecular Entanglement and Electrospinnability of Biopolymers
07:59

Molecular Entanglement and Electrospinnability of Biopolymers

Published on: September 3, 2014

14.8K

Downstream processing and structural confirmation of pullulan - A comprehensive review.

Ram Sarup Singh1, Navpreet Kaur1, Dhandeep Singh2

  • 1Carbohydrates and Protein Biotechnology Laboratory, Department of Biotechnology, Punjabi University, Patiala 147 002, Punjab, India.

International Journal of Biological Macromolecules
|March 30, 2022
PubMed
Summary
This summary is machine-generated.

This review details efficient, safe, and reproducible downstream processing for microbial polymer pullulan recovery. It covers solid-liquid and liquid-liquid separations, purification methods, and structural analysis techniques for Aureobasidium pullulans.

Keywords:
Aqueous phase systemDownstream processingOrganic solventsPullulan

More Related Videos

Automated Modular High Throughput Exopolysaccharide Screening Platform Coupled with Highly Sensitive Carbohydrate Fingerprint Analysis
12:02

Automated Modular High Throughput Exopolysaccharide Screening Platform Coupled with Highly Sensitive Carbohydrate Fingerprint Analysis

Published on: April 11, 2016

11.6K
Production, Crystallization and Structure Determination of C. difficile PPEP-1 via Microseeding and Zinc-SAD
13:34

Production, Crystallization and Structure Determination of C. difficile PPEP-1 via Microseeding and Zinc-SAD

Published on: December 30, 2016

11.7K

Related Experiment Videos

Last Updated: Sep 28, 2025

Molecular Entanglement and Electrospinnability of Biopolymers
07:59

Molecular Entanglement and Electrospinnability of Biopolymers

Published on: September 3, 2014

14.8K
Automated Modular High Throughput Exopolysaccharide Screening Platform Coupled with Highly Sensitive Carbohydrate Fingerprint Analysis
12:02

Automated Modular High Throughput Exopolysaccharide Screening Platform Coupled with Highly Sensitive Carbohydrate Fingerprint Analysis

Published on: April 11, 2016

11.6K
Production, Crystallization and Structure Determination of C. difficile PPEP-1 via Microseeding and Zinc-SAD
13:34

Production, Crystallization and Structure Determination of C. difficile PPEP-1 via Microseeding and Zinc-SAD

Published on: December 30, 2016

11.7K

Area of Science:

  • Biotechnology
  • Polymer Science
  • Microbial Processing

Background:

  • Pullulan is a microbial polymer produced commercially from Aureobasidium pullulans.
  • Efficient downstream processing is crucial for its industrial application.
  • Current methods require optimization for yield, safety, and reproducibility.

Purpose of the Study:

  • To review and analyze current methods for pullulan recovery and purification.
  • To highlight efficient techniques for solid-liquid and liquid-liquid separations.
  • To discuss perspectives on optimizing pullulan downstream processing.

Main Methods:

  • Filtration and centrifugation for cell biomass separation.
  • Organic solvent precipitation (ethanol, isopropanol) for pullulan recovery.
  • Aqueous two-phase systems and chromatographic techniques for purification.

Main Results:

  • Organic solvents are commonly used due to pullulan's insolubility.
  • Chromatography is effective but not cost-efficient for large-scale purification.
  • Aqueous two-phase systems offer an efficient purification alternative.

Conclusions:

  • Optimized solid-liquid and liquid-liquid separations are key to efficient pullulan recovery.
  • Aqueous two-phase systems present a promising, cost-effective purification strategy.
  • Further research into structural attributes aids process development.