Comparative microelectrode array data of the functional development of hPSC-derived and rat neuronal networks

Area of Science:

• Neuroscience
• Stem Cell Biology
• Computational Neuroscience

Background:

• In vitro models of neuronal development are crucial for understanding brain function and disease.
• Comparing human pluripotent stem cell (hPSC)-derived neurons with established animal models aids in validating new research tools.

Purpose of the Study:

• To present a comprehensive dataset of microelectrode array (MEA) recordings from maturing hPSC-derived and rat embryonic cortical neurons.
• To enable comparative analysis of functional development and network properties between human and rat neuronal cultures.
• To provide analysis code for reproducing key findings and facilitating further research.

Main Methods:

• Microelectrode array (MEA) recordings of spontaneous neuronal activity.
• Pharmacological response assessments of mature neuronal cultures.
• Analysis of spike times, waveforms, burst activity, and network synchronization metrics.

Main Results:

• The dataset captures extracellularly recorded spontaneous activity during in vitro maturation.
• Pharmacological responses of mature hPSC-derived and rat cortical cultures are included.
• Analysis code for spike sorting, burst detection, and network connectivity estimation is provided.

Conclusions:

• This dataset offers a valuable resource for investigating functional maturation of cortical neuronal networks.
• It facilitates the assessment of similarities and differences between hPSC-derived and rat cortical networks in vitro.
• The availability of raw data and analysis code supports experimental and computational neuroscience research.