Related Experiment Video
Updated: Sep 28, 2025
08:06
The Use of a β-lactamase-based Conductimetric Biosensor Assay to Detect Biomolecular Interactions
Published on: February 1, 2018
9.1K
A solid-state electrochemiluminescence aptasensor for β-lactoglobulin using Ru-AuNP/GNP/Naf nanocomposite-modified
Chitra Padmakumari Kurup1, Noor Faizah Mohd-Naim2, Minhaz Uddin Ahmed3
1Biosensors and Nanobiotechnology Laboratory, Integrated Science Building, Faculty of Science, Universiti Brunei Darussalam, Jalan Tungku Link, Gadong, 1410, BE, Brunei Darussalam.
Mikrochimica Acta
|March 31, 2022
Summary
A novel electrochemiluminescence (ECL) aptasensor was developed for sensitive detection of the milk allergen beta-lactoglobulin (β-LG). This cost-effective sensor utilizes a Ru-AuNPs/GNP/Naf nanocomposite for rapid and specific identification of β-LG.
Area of Science:
- Analytical Chemistry
- Biosensors
- Nanomaterials
Background:
- Accurate detection of milk allergens like beta-lactoglobulin (β-LG) is crucial for food safety and public health.
- Existing detection methods may lack sensitivity, speed, or cost-effectiveness.
- Electrochemical biosensors offer promising platforms for sensitive and selective analyte detection.
Purpose of the Study:
- To fabricate a novel electrochemiluminescence (ECL) aptasensor for the sensitive detection of β-LG.
- To utilize a Ru-AuNPs/GNP/Naf nanocomposite as a luminophore for enhanced sensing performance.
- To evaluate the aptasensor's performance in terms of sensitivity, specificity, stability, and applicability to real samples.
Main Methods:
- Fabrication of a nanocomposite using Rubpy32+-modified gold nanoparticles (Ru-AuNPs) and graphene nanoplatelets-Nafion (GNP/Naf).
- Immobilization of the nanocomposite onto a screen-printed electrode (SPCE) followed by chitosan coating.
- Covalent attachment of β-LG specific aptamers to the modified electrode surface using glutaraldehyde cross-linking.
Main Results:
- The ECL aptasensor exhibited a linear response to β-LG concentration in the range of 0.1 to 1000 pg/mL, with a low detection limit of 0.02 pg/mL.
- The sensor demonstrated high specificity, excellent reproducibility, and stability.
- The aptasensor achieved high recovery rates (92.5-112%) and low coefficients of variation (1.6-7.8%) when analyzing fortified milk samples.
Conclusions:
- The developed ECL aptasensor provides a sensitive, rapid, and cost-effective method for detecting the milk allergen β-LG.
- The integration of the Ru-AuNPs/GNP/Naf nanocomposite significantly enhances the sensor's performance.
- This approach holds potential for practical applications in food safety monitoring and allergen detection.
