Related Experiment Video
Updated: Sep 28, 2025
08:01
Stimulation of Notch Signaling in Mouse Osteoclast Precursors
Published on: February 28, 2017
8.0K
Periodic Heat Stress Licenses EMSC Differentiation into Osteoblasts via YAP Signaling Pathway Activation
Wentao Shi1, Zhe Wang2, Lu Bian1
1Jiangnan University Affiliated Hospital, Wuxi, Jiangsu Province 214122, China.
Stem Cells International
|March 31, 2022
Summary
Periodic heat stress enhances bone regeneration by promoting osteogenic differentiation in stem cells. This process involves YAP and TG2, crucial for cellular response to stress and tissue repair.
Area of Science:
- Biomaterials Science
- Stem Cell Biology
- Regenerative Medicine
Background:
- Large bone defect repair is a significant challenge in bone tissue engineering.
- Periodic heat stress has shown potential in stimulating osteogenesis.
- The thermal regulation of osteogenic differentiation in ectomesenchymal stem cells (EMSCs) requires further investigation.
Purpose of the Study:
- To investigate the direct effects of periodic heat stress on osteogenic differentiation of EMSCs.
- To elucidate the molecular mechanisms underlying heat-induced osteogenesis in EMSCs.
Main Methods:
- EMSCs were isolated from rat nasal respiratory mucosa.
- Cells were subjected to periodic heat stress (1 hour at 41°C every 7 days) during osteogenic differentiation.
- Key markers of osteogenic differentiation, including alkaline phosphatase (ALP) activity, osteogenic protein expression, and mineralization, were assessed.
- YAP nuclear translocation and transglutaminase 2 (TG2) expression were analyzed.
Main Results:
- Periodic heat stress significantly increased ALP activity, upregulated osteogenic-related proteins, and promoted EMSC mineralization.
- Increased YAP nuclear translocation was observed following heat stress.
- YAP knockdown inhibited heat-induced osteogenic differentiation.
- Heat stress led to increased expression and activity of TG2, correlated with YAP nuclear translocation.
Conclusions:
- Periodic heat stress is a viable strategy to enhance osteogenic differentiation of EMSCs.
- YAP plays a critical role in mediating the osteogenic response to periodic heat stress.
- The YAP-TG2 pathway is involved in regulating cellular proteostasis under thermal stress conditions, contributing to bone regeneration.
More Related Videos
Related Concept Videos
01:30Other Stress Responses in Bacteria
87
Bacteria have global regulatory systems that control several types of stress mechanisms. These include Pho regulon and the heat shock response, which are essential systems for environmental adaptation, such as nutrient limitation and proteotoxic stress. The Pho regulon and the heat shock response exemplify bacterial resilience, enabling rapid adaptation to fluctuating environmental conditions.Pho RegulonBacteria require phosphorus for essential cellular processes, including nucleic acid...
87
02:45Responses to Heat and Cold Stress
13.9K
Every organism has an optimum temperature range within which healthy growth and physiological functioning can occur. At the ends of this range, there will be a minimum and maximum temperature that interrupt biological processes.
13.9K
01:20The JAK-STAT Signaling Pathway
9.4K
Several cytokine receptors have tightly bound Janus kinase or JAK proteins attached at their cytosolic tail. Small signaling molecules such as cytokines, growth hormones, or prolactins bind to the cytokine receptors and initiate their dimerization. The dimerization brings the cytosolic JAKs together that trans-phosphorylate and activates each other. The activated JAKs now phosphorylate cytosolic tails of the cytokine receptors, which serve as binding sites for adaptor proteins such as SH2...
9.4K
01:17Role of ER in the Secretory Pathway
5.7K
Eukaryotic cells have a special pathway that enables communication between various intracellular membrane-bound compartments and also with the extracellular environment. This pathway is termed as the secretory pathway.
Components of the secretory pathway
About a third of proteins synthesized in the cell are sorted via the secretory route. They shuffle between different compartments in membrane-bound vesicles until they reach their final destination. The main intracellular compartments involved...
Components of the secretory pathway
About a third of proteins synthesized in the cell are sorted via the secretory route. They shuffle between different compartments in membrane-bound vesicles until they reach their final destination. The main intracellular compartments involved...
5.7K
01:31Osteoclasts in Bone Remodeling
3.3K
Osteoclasts are cells responsible for bone resorption and remodeling. They originate from hematopoietic progenitor cells present in the bone marrow. Numerous progenitor cells fuse to form multinucleated cells, each with 10-20 nuclei. A single osteoclast has a diameter of 150 to 200 µM. These cells have ruffled borders that break down the underlying bone tissue and release minerals such as calcium into the blood in bone resorption. Osteoclasts cling to bones with their ruffled edges during...
3.3K
01:09Thermal Stress
2.6K
If the temperature of an object is changed while it is prevented from expanding or contracting, the object is subjected to stress. The stress is compressive if the object expands in the absence of constraint and tensile if it contracts. This stress resulting from temperature change is known as thermal stress. It can be quite large and can cause damage. To avoid this stress, engineers may design components so they can expand and contract freely. For instance, on highways, gaps are deliberately...
2.6K