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Related Experiment Video

Updated: Sep 28, 2025

Engineered Lung Tissues Prepared from Decellularized Lung Slices
08:01

Engineered Lung Tissues Prepared from Decellularized Lung Slices

Published on: January 21, 2022

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Novel Decellularization Method for Tissue Slices.

Maria Narciso1,2, Anna Ulldemolins1, Constança Júnior1,2

  • 1Unitat de Biofísica i Bioenginyeria, Facultat de Medicina i Ciències de la Salut, Universitat de Barcelona, Barcelona, Spain.

Frontiers in Bioengineering and Biotechnology
|March 31, 2022
PubMed
Summary

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This summary is machine-generated.

A new method efficiently decellularizes tissue slices, like lungs, attached to glass slides. Sodium deoxycholate (SD) effectively removes cells while preserving extracellular matrix and scaffold integrity for tissue engineering.

Area of Science:

  • Biomaterials Science
  • Tissue Engineering
  • Cell Biology

Background:

  • Existing decellularization methods are optimized for whole organs or tissue blocks.
  • A need exists for protocols that allow side-by-side analysis of native and decellularized tissue slices.
  • Current methods are not ideal for preparing tissue slices for analysis while attached to a substrate.

Purpose of the Study:

  • To develop a fast and efficient decellularization protocol for tissue slices, specifically lung tissue, while attached to a glass slide.
  • To compare the efficacy of various decellularizing agents in cell removal and extracellular matrix preservation.
  • To evaluate the mechanical properties and biocompatibility of the resulting decellularized scaffolds.

Main Methods:

  • Tissue slices (primarily lung) were attached to glass slides.
Keywords:
biocompatibilitybioscaffold recellularizationdecellularizationextracellular matrixlungtissue slices

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  • Various decellularizing agents (CHAPS, Triton, ammonia hydroxide, SDS, SD) were tested.
  • Cell removal was assessed by DNA staining intensity.
  • Extracellular matrix components (collagen, elastin, laminin) were quantified via immunostaining.
  • Mechanical properties were analyzed using atomic force microscopy.
  • Biocompatibility was evaluated by recellularizing scaffolds with mesenchymal stromal cells.

    • Main Results:

    • Sodium deoxycholate (SD) proved optimal, achieving 6.5% remaining DNA signal and retaining 34% elastin.
    • Sodium dodecyl sulfate (SDS) effectively removed cells but significantly reduced elastin (6% remaining) and cell attachment (32%).
    • Collagen and laminin were preserved across tested agents; CHAPS, Triton, and ammonia hydroxide were insufficient for cell removal.
    • Decellularized scaffolds showed no significant mechanical changes and excellent biocompatibility (98% cell survival).
    • The SD protocol was validated on porcine and murine lungs, heart, kidney, and bladder tissues.

    Conclusions:

    • A novel, efficient protocol for decellularizing tissue slices attached to glass slides using sodium deoxycholate (SD) has been established.
    • This method preserves crucial extracellular matrix components and scaffold integrity, enabling biocompatible tissue engineering applications.
    • The protocol is adaptable for various tissues and scarce samples, offering new possibilities for disease research and regenerative medicine.