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ROR2 Downregulation Activates the MSX2/NSUN2/p21 Regulatory Axis and Promotes Dental Pulp Stem Cell Senescence

Xin He1, Zhan Yang2, Xiao-Yang Chu3

  • 1Department of Orthodontics, Beijing Stomatological Hospital, Capital Medical University School of Stomatology, Capital Medical University, Beijing, People's Republic of China.

Stem Cells (Dayton, Ohio)
|March 31, 2022
PubMed

Insights

Cellular senescence in dental pulp stem cells (DPSCs) is linked to ROR2 and MSX2. Targeting the ROR2/MSX2/NSUN2 pathway can inhibit DPSC aging and restore self-renewal capacity.

Area of Science:

  • Stem cell biology
  • Molecular and cellular biology
  • Regenerative medicine

Background:

  • Cellular senescence limits dental pulp stem cell (DPSC) research and applications.
  • ROR2 is implicated in DPSC senescence, but the regulatory mechanism is unclear.

Purpose of the Study:

  • To elucidate the mechanism of ROR2 regulation in DPSC senescence.
  • To identify potential targets for antagonizing DPSC aging.

Main Methods:

  • Investigated MSX2 expression in aging DPSCs.
  • Performed MSX2 depletion and ROR2 overexpression experiments.
  • Analyzed the ROR2/MSX2/NSUN2/p21 axis in DPSC senescence.

Main Results:

  • MSX2 expression increased in aging DPSCs.
  • MSX2 depletion inhibited DPSC senescence and restored self-renewal.
  • The ROR2/MSX2/NSUN2 axis was identified as a key regulator of DPSC aging.
  • ROR2 downregulation affected MSX2 protein levels by altering phosphorylation and ubiquitination.

Conclusions:

  • The ROR2/MSX2/NSUN2 axis plays a vital role in regulating DPSC senescence.
  • This pathway represents a potential therapeutic target for combating DPSC aging.
Keywords:
MSX2NSUN2ROR2dental pulp stem cellsenescence

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