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Related Experiment Video

Updated: Sep 28, 2025

Isolation of Cortical Microglia with Preserved Immunophenotype and Functionality From Murine Neonates

Isolation of Cortical Microglia with Preserved Immunophenotype and Functionality From Murine Neonates

Published on: January 30, 2014

Pain-resolving microglia.

George Sideris-Lampretsas¹, Marzia Malcangio¹

¹Wolfson Centre for Age-Related Diseases, King's College London, London, UK.

Science (New York, N.Y.)

|March 31, 2022

Summary

This summary is machine-generated.

A specific group of spinal cord microglia helps the body recover from long-lasting pain. These cells play a key role in pain relief and improving function after injury.

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Last Updated: Sep 28, 2025

Isolation of Cortical Microglia with Preserved Immunophenotype and Functionality From Murine Neonates

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Published on: July 25, 2022

Area of Science:

Neuroscience
Immunology
Pain Research

Background:

Persistent pain affects millions globally, often involving complex cellular mechanisms.
Microglia, the immune cells of the central nervous system, are increasingly recognized for their roles beyond inflammation.

Purpose of the Study:

To identify specific microglial subpopulations involved in the resolution of persistent pain.
To elucidate the mechanisms by which these microglia promote recovery.

Main Methods:

Single-cell RNA sequencing of spinal cord microglia.
In vivo pain models and genetic manipulation of microglial subsets.
Behavioral analysis of pain phenotypes and functional recovery.

Main Results:

An emergent subgroup of spinal cord microglia was identified.
This microglial subgroup was found to actively mediate recovery from persistent pain.
Depletion of this subgroup exacerbated pain persistence.

Conclusions:

Specific spinal cord microglia are crucial for resolving persistent pain.
Targeting this microglial subgroup may offer novel therapeutic strategies for chronic pain conditions.