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Related Concept Videos

Chromatin Immunoprecipitation- ChIP02:36

Chromatin Immunoprecipitation- ChIP

11.4K
Chromatin immunoprecipitation, or ChIP, is an antibody-based technique used to identify sites on DNA that bind to transcription factors of interest or histone proteins. It also helps determine the type of histone modifications such as acetylation, phosphorylation, or methylation.
Types of ChIP
ChIP can be divided into two types - X-ChIP and N-ChIP. X-ChIP involves in vivo cross-linking of histones and regulatory proteins to DNA, fragmenting the DNA by sonication, and isolating the protein-DNA...
11.4K

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Updated: Sep 28, 2025

Chromatin Immunoprecipitation in the Cnidarian Model System Exaiptasia diaphana
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Monitoring Chromatin Regulation in Planarians Using Chromatin Immunoprecipitation Followed by Sequencing (ChIP-seq).

Divya Sridhar1, Aziz Aboobaker2

  • 1Department of Zoology, University of Oxford, Oxford, UK.

Methods in Molecular Biology (Clifton, N.J.)
|April 1, 2022
PubMed
Summary

This study presents a new Chromatin Immunoprecipitation followed by sequencing (ChIP-seq) method for planarian cells. This technique aids in understanding the epigenetic mechanisms governing regeneration and stem cell pluripotency in animals.

Keywords:
ChromatinEpigenetic regulationFACSHistone modificationsImmunoprecipitationPlanarianPluripotency

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Area of Science:

  • Developmental Biology
  • Stem Cell Biology
  • Genomics

Background:

  • Planarians are a key model organism for studying animal regeneration and adult stem cells.
  • Recent advancements in molecular techniques have enhanced the study of whole-body regeneration and pluripotency.

Purpose of the Study:

  • To describe a novel Chromatin Immunoprecipitation followed by sequencing (ChIP-seq) protocol for planarian cells.
  • To enable whole-genome profiling of histone modifications for understanding epigenetic regulation.

Main Methods:

  • The protocol involves Fluorescence-Activated Cell Sorting (FACS) to isolate specific planarian cell populations.
  • Immunoprecipitation and library preparation are performed for next-generation sequencing.

Main Results:

  • The method allows for whole-genome profiling of histone modifications in planarian cells.
  • This provides insights into epigenetic mechanisms controlling development, pluripotency, and differentiation.

Conclusions:

  • This ChIP-seq protocol is a valuable addition to functional genomic approaches for studying planarian regeneration.
  • It facilitates a deeper understanding of the epigenetic basis of whole-body regeneration and stem cell biology.