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Updated: Sep 28, 2025

Exploring Sequence Space to Identify Binding Sites for Regulatory RNA-Binding Proteins
Published on: August 9, 2019
Dependence of click-SELEX performance on the nature and average number of modified nucleotides
Julia Siegl1, Olga Plückthun1, Günter Mayer1,2
1Chemical Biology & Chemical Genetics, Life and Medical Sciences (LIMES) Institute, University of Bonn Gerhard-Domagk-Str. 1 53121 Bonn Germany gmayer@uni-bonn.de.
Abstract:
The click-SELEX procedure enables the identification of nucleobase-modified aptamers in which chemical entities are introduced by a copper(i)-catalysed alkyne-azide 'click' reaction. Here we report on the impact of modified nucleobases on PCR conditions and the average amount of modified nucleobases on click-SELEX performance. We demonstrate click-SELEX being strongly dependent on which and on how many modifications are used. However, when using C3-GFP the number of modifications did not impact the overall success of the selection procedure.

