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Updated: Sep 28, 2025

Combining Non-reducing SDS-PAGE Analysis and Chemical Crosslinking to Detect Multimeric Complexes Stabilized by Disulfide Linkages in Mammalian Cells in Culture
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Combining Non-reducing SDS-PAGE Analysis and Chemical Crosslinking to Detect Multimeric Complexes Stabilized by Disulfide Linkages in Mammalian Cells in Culture

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Streamlining the Characterization of Disulfide Bond Shuffling and Protein Degradation in IgG1 Biopharmaceuticals

Jill Coghlan1, Alexander Benet1, Preethi Kumaran1

  • 1Department of Pharmaceutical Sciences, College of Pharmacy, University of Michigan, Ann Arbor, MI, United States.

Frontiers in Bioengineering and Biotechnology
|April 1, 2022
PubMed

Keywords:
IgG1 therapeuticsLC-MS/MSbiosimilarsdisulfide bond shufflingprotein degradation

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