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Updated: Sep 28, 2025
Diagnostic Necropsy and Selected Tissue and Sample Collection in Rats and Mice
Published on: August 7, 2011
A guide to post-mortem examination procedure in mouse models
Alexandra Rodrigues1, Lynn Beresford1, Cheryl L Scudamore2
1Mary Lyon Centre, MRC Harwell, UK.
Abstract:
The main objective of a post mortem is to identify, at a macroscopic level, any anatomical features that characterise mutant or sick mice and to describe lesions contributing to morbidity and mortality. Tissues collected for subsequent examination require appropriate handling and preservation to prevent deterioration. Therefore, efficient routine procedures are essential to facilitate histology and to ensure high-quality samples. In addition, optimised techniques minimise data loss from damaged samples reducing the numbers of animals used and supporting the 3Rs principle of reduction. Here, we describe an optimised method for tissue collection in the mouse. Training tips and points for caution are included.
Insights
This study presents an optimized mouse tissue collection method for post-mortem examinations. This technique ensures high-quality samples for histology, reducing animal use and supporting ethical research principles.
Area of Science:
- Veterinary Pathology
- Laboratory Animal Science
- Histology
Background:
- Accurate post-mortem examination in mice is crucial for identifying disease indicators.
- Proper tissue handling and preservation are essential for reliable histological analysis.
- Current methods may lead to sample degradation and data loss, impacting research integrity.
Purpose of the Study:
- To describe an optimized, standardized method for mouse tissue collection post-mortem.
- To improve the quality of samples for subsequent histological examination.
- To support the ethical reduction of animal usage in research.
Main Methods:
- Detailed description of an optimized tissue collection protocol for mice.
- Inclusion of practical training tips for researchers.
- Highlighting critical points for caution during sample acquisition.
Main Results:
- The optimized method facilitates efficient routine procedures for histology.
- High-quality tissue samples are ensured, minimizing deterioration.
- Reduced sample damage contributes to minimizing data loss.
Conclusions:
- The described optimized method enhances the quality and reliability of post-mortem mouse tissue analysis.
- Implementation of this protocol supports the 3Rs principles by reducing animal numbers.
- This standardized approach is vital for accurate pathological assessment and reproducible research.
