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Updated: Sep 28, 2025
A Protocol for Analyzing Hepatitis C Virus Replication
Published on: June 26, 2014
A visual method for determination of hepatitis C virus RNAs based on a 3D nanocomposite prepared from graphene
Yifei Li1, Jingwen Li2, Ya Cao3
1CAS Key Lab of Bio-Medical Diagnostics, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou, 215163, PR China.
Abstract:
Sensitive and specific assay of hepatitis C virus (HCV) RNA has a great clinical significance for the diagnosis of hepatitis C in window period. A colorimetric assay is described for determination of the RNA of hepatitis C virus. The method is based on the in situ synthesis of graphene quantum dot-silver nano-composites (GQD/Ag NCs), magnetic separation and catalyzed hairpin assembly reaction (CHA). The silver component of GQD/Ag NCs react with H2O2 generated by glucose oxidase, which are induced via CHA. Then the light-yellow solution turns into colorless transparent in the presence of the target RNAs. The method has a linear response in the 25-500 pM RNA concentration range, and the detection limit is 24.84 pM. The method is simple, stable and sensitive and might be extend to the assay of other RNA viruses. The retro-transcription free and visual distinguishable properties endow this strategy a promising application in the screening of HCV.
