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Updated: Sep 28, 2025

Purification of Biotinylated Cell Surface Proteins from Rhipicephalus microplus Epithelial Gut Cells
Published on: July 23, 2017
Comparative analyses of the mitochondrial genomes of the cattle tick Rhipicephalus microplus clades A and B from
Yuan-Ping Deng1, Jia-Ning Yi1, Yi-Tian Fu1
1Research Center for Parasites & Vectors, College of Veterinary Medicine, Hunan Agricultural University, Changsha, 410128, Hunan Province, China.
Abstract:
The cattle tick (Rhipicephalus microplus) is one of the most common ticks parasitizing livestock, causing diseases as the vector of pathogens. In this study, we amplified and sequenced the complete mitochondrial (mt) genome of R. microplus from Hainan province of China and compared it with that of R. microplus from Guizhou province of China. The mt genome sequence of R. microplus from Hainan isolate was 15,163 bp in size, which was significantly longer (299 bp) than R. microplus from Guizhou isolate. Nucleotide sequence difference in the entire mt genome except for non-coding region was 5.6% between R. microplus from Hainan and Guizhou isolates. For the 13 protein-coding genes, this comparison revealed the sequence differences of nucleotide (3.8-10.1%) and amino acid (1.2-17.3%). Phylogenetic analysis of R. microplus indicated that R. microplus from Hainan isolate clustered in clade A, and R. microplus from Guizhou isolate clustered in clade B. Taken together, the findings support the recent proposal the existence of two lineages (clades A and B) of R. microplus in China.
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