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Updated: Sep 28, 2025

Assessment of DNA Contamination in RNA Samples Based on Ribosomal DNA

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Published on: January 22, 2018

28S rRNA sequences for Linguatula spp.

Shokoofeh Shamsi¹, Xiaocheng Zhu^1,2, Ali Halajian^3,4

¹School of Agricultural, Environmental and Veterinary Sciences, Charles Sturt University, Wagga Wagga, NSW, 2678, Australia.

Parasitology Research

|April 1, 2022

Summary

This summary is machine-generated.

Differentiating Linguatula parasite species is challenging. This study reveals that 28S rRNA gene sequencing offers greater genetic diversity for distinguishing Linguatula species compared to 18S rRNA, aiding accurate identification.

Keywords:

Linguatula nuttalli Linguatula serrata Molecular sequences Pentastomida Phylogeny

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RNA Next-Generation Sequencing and a Bioinformatics Pipeline to Identify Expressed LINE-1s at the Locus-Specific Level

RNA Next-Generation Sequencing and a Bioinformatics Pipeline to Identify Expressed LINE-1s at the Locus-Specific Level

Published on: May 19, 2019

Area of Science:

Parasitology
Molecular Biology
Phylogenetics

Background:

Accurate identification of Linguatula species (Pentastomida) is hindered by morphological variations across developmental stages.
Existing molecular data in GenBank is limited, primarily to 18S rRNA and Cox1 sequences.
The utility of 28S ribosomal RNA (rRNA) for pentastomid phylogenetic studies is under investigation.

Purpose of the Study:

To present 28S rRNA gene sequences for two Linguatula species: L. serrata and L. nuttalli.
To evaluate the effectiveness of 28S rRNA in differentiating between Linguatula species.
To compare the genetic diversity of 28S rRNA with 18S rRNA and Cox1 markers.

Main Methods:

Sequencing of the 28S rRNA gene from well-identified voucher specimens of L. serrata (Australia) and L. nuttalli (South Africa).
Phylogenetic analysis of obtained 28S rRNA sequences.
Comparison of interspecific and intraspecific variation with existing 18S rRNA and Cox1 sequence data.

Main Results:

Successfully obtained and analyzed 28S rRNA gene sequences for L. serrata and L. nuttalli.
Identified a 6% sequence difference between L. serrata and L. nuttalli using 28S rRNA, with low intraspecific variation.
Observed interspecific differences of 0.23% for 18S rRNA and 13% for Cox1 from the same specimens.

Conclusions:

28S rRNA exhibits greater genetic diversity than 18S rRNA, facilitating improved species differentiation within the Linguatula genus.
The discriminatory power of 28S rRNA is comparable to that of Cox1 for Linguatula species identification.
Emphasizes the need for cautious interpretation of records lacking robust morphological or molecular data and highlights the importance of integrated analyses using multiple markers, hosts, developmental stages, and geographical regions.