Exposure to clothianidin and predators increases mortality for heptageniidae

Neonicotinoids are a class of insecticide with global impacts on natural environments. Due to their high solubility, they are frequently found in stream ecosystems where they have the potential to impact non-target biota. While environmental concentrations are generally below lethal levels for most organisms, there are concerns that sublethal exposures can impact aquatic insects, particularly mayflies, which are highly sensitive to neonicotinoids. Because sublethal doses of neonicotinoids can reduce mobility in mayflies, exposure could indirectly increase mortality due to predation by impairing their ability to avoid initial detection or escape predators. We examined whether exposure to the neonicotinoid clothianidin at a concentration below the 96-h EC50 (7.5 µg/L), would increase the predation risk of Stenacron and Stenonema mayfly nymphs by larval southern two-lined salamanders (Eurycea cirrigera) or eastern dobsonfly nymphs (Corydalus cornutus) using a controlled laboratory experiment. For Stenacron, we found significant interactive effects between pesticide and dobsonfly exposure that increased the hazard ratio (HR). The HR assesses risk relative to a control population, in this case mayflies in similar experimental conditions but without exposure to neonicotinoids or predators. With the addition of clothianidin, the HR of mayflies exposed to a dobsonfly nymph significantly increased from 1.8 to 6.2 while the HR for those exposed to salamanders increased from 7.6 to 12.5. For Stenonema, the HR initially decreased due to dobsonfly exposure (1 to 0.3) but increased when clothianidin and dobsonflies were combined (0.3 to 1.6). Our study shows that aquatic exposure to clothianidin can increase mortality for aquatic insects through predator pressure. Such indirect effects associated with neonicotinoid exposure warrant further investigation to expand our understanding of pesticide impacts to aquatic systems.