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Circular RNA circGSE1 promotes angiogenesis in ageing mice by targeting the miR-323-5p/NRP1 axis

Jiacong Qiu1,2,3, Rencong Chen1,2,3, Lei Zhao1,2,3

  • 1Division of Vascular Surgery, The First Affiliated Hospital, Sun Yat-Sen University, Guangzhou 510080, Guangdong, China.

Aging
|April 2, 2022
PubMed

Insights

Circular RNA circGSE1 promotes blood vessel formation in aging endothelial cells by regulating the miR-323-5p/NRP1 pathway, offering a potential therapeutic target for age-related cardiovascular issues.

Area of Science:

  • Cardiovascular Biology
  • Molecular Biology
  • Aging Research

Background:

  • Endothelial cells (ECs) are crucial in cardiovascular health, with aging impairing their function and contributing to disease.
  • Circular RNAs (circRNAs) are implicated in cardiovascular diseases, but their role in age-related endothelial dysfunction and angiogenesis remains largely unknown.

Purpose of the Study:

  • To identify a functional circRNA regulating angiogenesis in aging ECs.
  • To elucidate the underlying molecular mechanism of this circRNA in promoting endothelial angiogenesis during aging.

Main Methods:

  • CircRNA sequencing to identify differentially expressed circRNAs in old (OECs) versus young (YECs) endothelial cells.
  • In vitro assays (proliferation, migration, tube formation) to assess circGSE1 function in OECs.
  • In vivo mouse model (femoral artery ligation) to evaluate circGSE1's effect on angiogenesis in aging mice.

Main Results:

  • circGSE1 was significantly downregulated in OECs compared to YECs.
  • Overexpression of circGSE1 enhanced proliferation, migration, and tube formation in OECs in vitro.
  • circGSE1 promoted blood flow recovery and angiogenesis in ischemic limbs of aging mice, acting via the miR-323-5p/NRP1 axis.

Conclusions:

  • circGSE1 plays a vital role in promoting endothelial angiogenesis during aging.
  • The circGSE1/miR-323-5p/NRP1 axis is a key mechanism regulating angiogenesis in aging ECs.
  • This axis represents a promising therapeutic target for enhancing angiogenesis in aging-related cardiovascular conditions.

Keywords:
NRP1ageingangiogenesiscircGSE1endothelial cell

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